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Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution

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Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution

dc.contributor.author	Carda Diéguez, Miguel
dc.contributor.author	Ghai, Rohit
dc.contributor.author	Rodríguez Valera, Francisco Eduardo
dc.contributor.author	Amaro González, Carmen
dc.date.accessioned	2019-05-07T13:25:11Z
dc.date.available	2019-05-07T13:25:11Z
dc.date.issued	2017
dc.identifier.citation	Carda Diéguez, Miguel Ghai, Rohit Rodríguez Valera, Francisco Eduardo Amaro González, Carmen 2017 Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution Microbiome 5 162 1 15
dc.identifier.uri	http://hdl.handle.net/10550/70101
dc.description.abstract	BACKGROUND: Fish skin mucosal surfaces (SMS) are quite similar in composition and function to some mammalian MS and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. We aimed to test this hypothesis by searching for metagenomic and genomic evidences in the SMS-microbiome of a model fish species (Anguilla Anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the water microbiome (W-microbiome) surrounding the host. RESULTS: Remarkably, potentially pathogenic Vibrio monopolized wild eel SMS-microbiome from natural ecosystems, Vibrio anguillarum/Vibrio vulnificus and Vibrio cholerae/Vibrio metoecus being the most abundant ones in SMS from estuary and lake, respectively. Functions encoded in the SMS-microbiome differed significantly from those in the W-microbiome and allowed us to predict that successful mucus colonizers should have specific genes for (i) attachment (mainly by forming biofilms), (ii) bacterial competence and communication, and (iii) resistance to mucosal innate immunity, predators (amoeba), and heavy metals/drugs. In addition, we found several mobile genetic elements (mainly integrative conjugative elements) as well as a series of evidences suggesting that bacteria exchange DNA in SMS. Further, we isolated and sequenced a V. metoecus strain from SMS. This isolate shares pathogenicity islands with V. cholerae O1 from intestinal infections that are absent in the rest of sequenced V. metoecus strains, all of them from water and extra-intestinal infections. CONCLUSIONS: We have obtained metagenomic and genomic evidence in favor of the hypothesis on the role of fish mucosal surfaces as a specialized habitat selecting microbes capable of colonizing and persisting on other comparable mucosal surfaces, e.g., the human intestine.
dc.language.iso	eng
dc.relation.ispartof	Microbiome, 2017, vol. 5, num. 162, p. 1-15
dc.subject	Bacteris patògens
dc.subject	Genòmica
dc.title	Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution
dc.type	journal article	es_ES
dc.date.updated	2019-05-07T13:25:11Z
dc.identifier.doi	10.1186/s40168-017-0376-1
dc.identifier.idgrec	125494
dc.rights.accessRights	open access	es_ES