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Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase

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Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase

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dc.contributor.author Awang-Kechik, Noor-Hidayah es
dc.contributor.author Ahmad, Rohana es
dc.contributor.author Doustjalali, Saeid-Reza es
dc.contributor.author Sabet, Negar-Shafiei es
dc.contributor.author Abd Rahman, Aida Nur Ashikin es
dc.date.accessioned 2019-07-02T09:21:05Z
dc.date.available 2019-07-02T09:21:05Z
dc.date.issued 2019 es
dc.identifier.citation Awang-Kechik, Noor-Hidayah ; Ahmad, Rohana ; Doustjalali, Saeid-Reza ; Sabet, Negar-Shafiei ; Abd Rahman, Aida Nur Ashikin. Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase. En: Journal of Clinical and Experimental Dentistry, 11 3 2019: 269-274 es
dc.identifier.uri https://hdl.handle.net/10550/70625
dc.description.abstract The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach. A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little?s Irregularity Index at 6-months retention phase. 146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase. Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment. es
dc.title Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/jced.55546 es
dc.type.hasVersion VoR es_ES

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