This study aimed to investigate the influence of hesperidin application on the bonding of resin-modified glass ionomer-based restorative material to dentin treated with oxygen-induced endodontic irrigants. One hundred human permanent molars were categorized into five groups (n= 20/group), treated with various irrigants as follows: Group C; distilled water (control group), Group SH; 5.25% NaOCl, Group SH+H; 5.25% NaOCl + 5 % hesperidin, Group HP; 10% H2O2, Group HP+H; 10% H2O2 + 5 % hesperidin. Specimens were bonded with RMGI based restorative material. For each group, half of the specimens were evaluated for µSBS by a universal testing machine and the other half for dentin ion uptake by EDX. Additional ten specimens (n=2/per group) were prepared for the micro-morphological analysis under SEM. Hesperidin groups improved the µSBS, with a significant effect for HP+H group (p<. 05). Dentin ion uptake was significantly (p<. 05) improved in hesperidin groups. In conclusion, Application of hesperidin in conjunction with RMGI based restorative material improved the dentin bond strength and ion uptake; this could be a promising approach to aid dental practitioners in their decisions, regarding which restorative material to use especially in caries susceptible patients.