The increasing demand of tooth bleaching has also increased the need of researches focusing on the durability of the resultant color. The aim of this study was to evaluate the effect of a system based on calcium silicate, sodium phosphate and sodium monofluorophosphate (Regenerate?) on color maintenance of bleached enamel considering two waiting times for the contact with a cola drink. This in vitrostudy was performed on bovine enamel specimens (n = 100), bleached with 35% H2O2 and treated with: G1 Distilled water; G2 Artificial saliva; G3 RegenerateTM Serum and Toothpaste; G4 RegenerateTM Toothpaste; G5 RegenerateTM Serum. The groups G3, G4 and G5 received one application of the respective products for 3 min, in 3 consecutive days. The color parameters (?E, L*, a*, b*) were evaluated by spectrophotometry before and after bleaching and after surface treatments and immersion (15min) in cola drink, on the waiting times of 24 hours (T1) and 7 days (T2). The effect of surface treatments and waiting times was evaluated by Two-Way ANOVA, Kruskal-Wallis, Wilcoxon and T test (p<0.05). There was no significant effect of the surface treatments (p=0.57), waiting times (p=0.97) and their interaction (p=0.47) considering ?E. The analysis of repeated measures of the color coordinates L*, a* and b* showed a decrease of chromaticity (a*, b*) for G3, G4 and G5 and an increase of lightness (L*) for G5, after immersion in cola drink, suggesting some protection against bleached enamel pigmentation. RegenerateTM has a potential protective effect on bleached enamel color maintenance. The waiting times of 24 hours and 7 days for the contact with the cola drink did not influence bleached enamel color maintenance.