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Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing

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Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing

dc.contributor.author	López-Galiano, María José
dc.contributor.author	Sentandreu, Vicente
dc.contributor.author	Martínez Ramírez, Amparo
dc.contributor.author	Rausell Segarra, Carolina
dc.contributor.author	Real García, María Dolores
dc.contributor.author	Camañes, Gemma
dc.contributor.author	Ruiz-Rivero, Omar
dc.contributor.author	Crespo Salvador, Óscar
dc.contributor.author	García Robles, Inmaculada
dc.date.accessioned	2019-07-22T09:14:00Z
dc.date.available	2019-07-22T09:14:00Z
dc.date.issued	2019
dc.identifier.citation	López-Galiano, María José Sentandreu, Vicente Martínez Ramírez, Amparo Rausell Segarra, Carolina Real García, María Dolores Camañes, Gemma Ruiz-Rivero, Omar Crespo Salvador, Óscar García Robles, Inmaculada 2019 Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing Genes 10 6 475
dc.identifier.uri	https://hdl.handle.net/10550/70921
dc.description.abstract	Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to validate high-throughput expression analysis data, confirming the expression profiles of 10 out of 11 randomly selected miRNAs. Most of the differentially expressed miRNAs were stress-specific, except for sly-miR167c-3p upregulated in B. cinerea and P. syringae infection, sly-newmiR26-3p upregulated in drought and Hx treatment samples, and sly-newmiR33-3p, sly-newmiR6-3p and sly-newmiR8-3p differentially expressed both in biotic and abiotic stresses. From mature miRNAs sequences of the 41 stress-responsive miRNAs 279 targets were predicted. An inverse correlation between the expression profiles of 4 selected miRNAs (sly-miR171a, sly-miR172c, sly-newmiR22-3p and sly-miR167c-3p) and their target genes (Kinesin, PPR, GRAS40, ABC transporter, GDP and RLP1) was confirmed by RT-qPCR. Altogether, our analysis of miRNAs in different biotic and abiotic stress conditions highlight the interest to understand the functional role of miRNAs in tomato stress response as well as their putative targets which could help to elucidate plants molecular and physiological adaptation to stress.
dc.language.iso	eng
dc.relation.ispartof	Genes, 2019, vol. 10, num. 6, p. 475
dc.subject	Tomàquets
dc.subject	Estrès
dc.title	Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing
dc.type	journal article	es_ES
dc.date.updated	2019-07-22T09:14:01Z
dc.identifier.doi	10.3390/genes10060475
dc.identifier.idgrec	133614
dc.rights.accessRights	open access	es_ES