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The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4

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The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4

dc.contributor.author	Begley, Victoria
dc.contributor.author	Corzo, Daniel
dc.contributor.author	Jordán Plá, Antonio
dc.contributor.author	Cuevas-Bermúdez, Abel
dc.contributor.author	De Miguel-Jiménez, Lola
dc.contributor.author	Pérez-Aguado, David
dc.contributor.author	Machuca-Ostos, Mercedes
dc.contributor.author	Navarro, Francisco
dc.contributor.author	Chávez, María José
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Chávez, Sebastián
dc.date.accessioned	2019-10-28T17:49:50Z
dc.date.available	2019-10-28T17:49:50Z
dc.date.issued	2019
dc.identifier.citation	Begley, Victoria Corzo, Daniel Jordán Plá, Antonio Cuevas-Bermúdez, Abel De Miguel-Jiménez, Lola Pérez-Aguado, David Machuca-Ostos, Mercedes Navarro, Francisco Chávez, María José Pérez Ortín, José Enrique Chávez, Sebastián 2019 The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4 Nucleic Acids Research 47 18 9524 9541
dc.identifier.uri	https://hdl.handle.net/10550/71819
dc.description.abstract	Co-transcriptional imprinting of mRNA by Rpb4 and Rpb7 subunits of RNA polymerase II (RNAPII) and by the Ccr4-Not complex conditions its posttranscriptional fate. In turn, mRNA degradation factors like Xrn1 are able to influence RNAPII-dependent transcription, making a feedback loop that contributes to mRNA homeostasis. In this work, we have used repressible yeast GAL genes to perform accurate measurements of transcription and mRNA degradation in a set of mutants. This genetic analysis uncovered a link from mRNA decay to transcription elongation.We combined this experimental approach with computational multi-agent modelling and tested different possibilities of Xrn1 and Ccr4 action in gene transcription. This double strategy brought us to conclude that both Xrn1-decaysome and Ccr4-Not regulate RNAPII elongation, and that they do it in parallel. We validated this conclusion measuring TFIIS genome-wide recruitment to elongating RNAPII. We found that xrn1Δ and ccr4Δ exhibited very different patterns of TFIIS versus RNAPII occupancy, which confirmed their distinct role in controlling transcription elongation. We also found that the relative influence of Xrn1 and Ccr4 is different in the genes encoding ribosomal proteins as compared to the rest of the genome.
dc.language.iso	eng
dc.relation.ispartof	Nucleic Acids Research, 2019, vol. 47, num. 18, p. 9524-9541
dc.subject	RNA
dc.subject	Genomes
dc.title	The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4
dc.type	journal article	es_ES
dc.date.updated	2019-10-28T17:49:51Z
dc.identifier.doi	10.1093/nar/gkz660
dc.identifier.idgrec	134949
dc.rights.accessRights	open access	es_ES