Splinting of the implants might improve the active tactile sensibility (ATS) of the pontic area due to cumulative effect of Osseo perception of two retainers; on the other hand, due to the lack of any supporting implant in the axis of occlusal force for the pontic area, ATS might be lower for this portion of FPDs. we evaluated the active tactile sensibility of natural teeth and three-unit implant-supported FPDs. The ATS of posterior 3-unit implant-supported FPD and contralateral teeth was measured in 50 patients, in a random order blinded to patients and assessor, carried out at two sessions. Based on the experimental range of 0 to 70 um, the sigmoid shape of psychometric curve was estimated to locate the 50% values as the ATS thresholds for each tooth or implant. Data were analyzed using unpaired t-tests. The ATS of the teeth and implants differed significantly and compared to teeth, implants exhibited significantly higher ATS thresholds in all the groups. The results of independent t-test showed the highest difference in the means of ATS between the pontic and the first molar tooth. Based on the equivalence testing approach, the 95% CIs indicated that the differences were clinically significant only in the Pontic/First Molar group. In multi-unit implant-supported prostheses the tactile perception of the prosthesis that are placed on fixtures is similar to the natural teeth. In pontic areas there are significant statistical and clinical differences, with much lower tactile sensibility in pontics compared to the natural teeth.