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Clickable poly-L-lysine for the formation of biorecognition surfaces
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Clickable poly-L-lysine for the formation of biorecognition surfaces
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| Di Iorio, Daniele; Martí Morant, Almudena; Koeman, Sander; Huskens, Jurriaan | |||
| Aquest document és un/a article, creat/da en: 2019 | |||
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| Biomolecules are immobilized onto surfaces employing the fast and stable adsorption of poly-L-lysine (PLL) polymers and the versatile copper-free click chemistry reactions. This method provides the combined advantages of versatile surface adsorption with density control using polyelectrolytes and of the covalent and orthogonal immobilization of biomolecules with higher reaction rates and improved yields of click chemistry. Using DNA attachment as a proof of concept, control over the DNA probe density and applicability in electrochemical detection are presented. | |||
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