This study aimed to determine the esthetic proportion of six natural upper anterior teeth in northeastern Thai population as well as the level of esthetic satisfaction of distinct tooth proportions. Front-view photographs were taken from participants living in the Northeastern Thailand (n=140) of the 18-35 years of age. Computerized program was used for measuring the size of each tooth. All subjects also scored the satisfaction level of different photographs portraying 4 proportions of anterior teeth (golden proportion, 70% RED, 80% RED, and an increasing proportion). We found that proportion of lateral-to-central incisor and canine-to-lateral incisor were 0.72 and 0.80, respectively on both sides. The proportions increased in mesio-distal direction. Our reported ratios were statistically different (P<0.05) from the golden proportion, golden percentage, and 70% RED. However, the ratio of lateral-to-central incisor, but not the canine-to-lateral ratio, was significantly different (P<0.05) when compared to 80% RED. Esthetic satisfaction level of 4 tooth proportions among northeastern Thais was not statistically different (P=0.054). An increasing proportion of upper anterior teeth in the northeastern Thai subpopulation was found. No difference of esthetic satisfaction of 4 different tooth proportions among Thai laypersons warrants further study.