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Optical sectioning microscopy through single-shot Lightfield protocol
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Optical sectioning microscopy through single-shot Lightfield protocol
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| Sánchez Ortiga, Emilio; Scrofani, G.; Saavedra Tortosa, Genaro; Martínez Corral, Manuel | |||
| Aquest document és un/a article, creat/da en: 2020 | |||
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| Optical sectioning microscopy is usually performed by means of a scanning, multi-shot procedure in combination with non-uniform illumination. In this paper, we change the paradigm and report a method that is based in the light field concept, and that provides optical sectioning for 3D microscopy images after a single-shot capture. To do this we fi rst capture multiple orthographic perspectives of the sample by means of Fourier-domain integral microscopy (FiMic). The second stage of our protocol is the application of a novel refocusing algorithm that is able to produce optical sectioning in real time, and with no resolution worsening, in the case of sparse f luorescent samples.We provide the theoretical derivation of the algorithm, and demonstrate its utility by applying it to simulations and to experimental data. | |||
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