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A major QTL located in chromosome 8 of Cucurbita moschata is responsible for resistance to tomato leaf curl New Delhi virus (ToLCNDV)

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A major QTL located in chromosome 8 of Cucurbita moschata is responsible for resistance to tomato leaf curl New Delhi virus (ToLCNDV)

dc.contributor.author	Sáez, Cristina
dc.contributor.author	Martínez, Cecilia
dc.contributor.author	Montero Pau, Javier
dc.contributor.author	Esteras, Cristina
dc.contributor.author	Sifres, Alicia
dc.contributor.author	Blanca, José
dc.contributor.author	Ferriol Molina, María
dc.contributor.author	López, Carmelo
dc.contributor.author	Picó, Belén
dc.date.accessioned	2020-04-03T10:30:34Z
dc.date.available	2020-04-03T10:30:34Z
dc.date.issued	2020
dc.identifier.citation	Sáez, Cristina Martínez, Cecilia Montero Pau, Javier Esteras, Cristina Sifres, Alicia Blanca, José Ferriol Molina, María López, Carmelo Picó, Belén 2020 A major QTL located in chromosome 8 of Cucurbita moschata is responsible for resistance to tomato leaf curl New Delhi virus (ToLCNDV) Frontiers In Plant Science 11 207 1 18
dc.identifier.uri	https://hdl.handle.net/10550/73767
dc.description.abstract	Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite whitefly transmitted begomovirus, responsible since 2013 of severe damages in cucurbit crops in Southeastern Spain. Zucchini (Cucurbita pepo) is the most affected species, but melon (Cucumis melo) and cucumber (Cucumis sativus) are also highly damaged by the infection. The virus has spread across Mediterranean basin and European countries, and integrated control measures are not being enough to reduce economic losses. The identification of resistance genes is required to develop resistant cultivars. In this assay, we studied the inheritance of the resistance to ToLCNDV previously identified in two Cucurbita moschata accessions. We generated segregating populations crossing both resistant pumpkins, an American improved cultivar Large Cheese (PI 604506) and an Indian landrace (PI 381814), with a susceptible C. moschata genotype (PI 419083). The analysis of symptoms and viral titers of all populations established the same monogenic recessive genetic control in both resistant accessions, and the allelism tests suggest the occurrence of alleles of the same locus. By genotyping with a single nucleotide polymorphism (SNP) collection evenly distributed along the C. moschata genome, a major quantitative trait locus (QTL) was identified in chromosome 8 controlling resistance to ToLCNDV. This major QTL was also confirmed in the interspecific C. moschata × C. pepo segregating populations, although C. pepo genetic background affected the resistance level. Molecular markers here identified, linked to the ToLCNDV resistance locus, are highly valuable for zucchini breeding programs, allowing the selection of improved commercial materials. The duplication of the candidate region within the C. moschata genome was studied, and genes with paralogs or single-copy genes were identified. Its synteny with the region of chromosome 17 of the susceptible C. pepo revealed an INDEL including interesting candidate genes. The chromosome 8 candidate region of C. moschata was also syntenic to the region in chromosome 11 of melon, previously described as responsible of ToLCNDV resistance. Common genes in the candidate regions of both cucurbits, with high- or moderate-impact polymorphic SNPs between resistant and susceptible C. moschata accessions, are interesting to study the mechanisms involved in this recessive resistance.
dc.language.iso	eng
dc.relation.ispartof	Frontiers In Plant Science, 2020, vol. 11, num. 207, p. 1-18
dc.subject	Virus
dc.subject	Plantes Malalties
dc.title	A major QTL located in chromosome 8 of Cucurbita moschata is responsible for resistance to tomato leaf curl New Delhi virus (ToLCNDV)
dc.type	journal article	es_ES
dc.date.updated	2020-04-03T10:30:35Z
dc.identifier.doi	10.3389/fpls.2020.00207
dc.identifier.idgrec	137528
dc.rights.accessRights	open access	es_ES