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Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

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Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

dc.contributor.author	Zueva, Tatiana
dc.contributor.author	Morchón, Rodrigo
dc.contributor.author	Carretón, Elena
dc.contributor.author	Montoya-Alonso, J. A.
dc.contributor.author	Santana, Alexis
dc.contributor.author	Bargues Castelló, María Dolores
dc.contributor.author	Mas-Coma, S.
dc.contributor.author	Rodríguez-Barbero, Alicia
dc.contributor.author	Simón, Fernando
dc.date.accessioned	2020-05-27T07:28:24Z
dc.date.available	2020-05-27T07:28:24Z
dc.date.issued	2019
dc.identifier.citation	Zueva, Tatiana Morchón, Rodrigo Carretón, Elena Montoya-Alonso, J. A. Santana, Alexis Bargues Castelló, María Dolores Mas-Coma, S. Rodríguez-Barbero, Alicia Simón, Fernando 2019 Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms Parasites & Vectors 12 315
dc.identifier.uri	https://hdl.handle.net/10550/74722
dc.description.abstract	Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis (Dirofilaria immitis). The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. Methods The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. In both cases non-stimulated cultures were used as controls. Results First, we demonstrated that worms obtained from the dog treated with doxycycline showed a significantly lower amount of Wolbachia (less than 60%) than worms removed from the untreated dog. Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. None of the D. immitis extracts modified the expression of sFlt. tDiSA extract was able to modify the expression of the endoglins, significantly decreasing the expression of the pro-angiogenic mEndoglin and increasing the anti-angiogenic sEndoglin. The formation of pseudocapillaries was negatively influenced by tDiSA, which reduced the organization and number of cellular connections. Conclusions The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro. The capacity of stimulation may be linked to the amount of Wolbachia present in the antigenic extracts.
dc.language.iso	eng
dc.relation.ispartof	Parasites & Vectors, 2019, vol. 12, num. 315
dc.subject	Parasitologia veterinària
dc.subject	Angiogènesi
dc.title	Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms
dc.type	journal article	es_ES
dc.date.updated	2020-05-27T07:28:25Z
dc.identifier.doi	10.1186/s13071-019-3570-0
dc.identifier.idgrec	139102
dc.rights.accessRights	open access	es_ES