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Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016-2017) in Valencia (Spain)

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Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016-2017) in Valencia (Spain)

dc.contributor.author	Santiso Bellón, Cristina
dc.contributor.author	Randazzo, Walter
dc.contributor.author	Pérez Cataluña, Alba
dc.contributor.author	Vila Vicent, Susana
dc.contributor.author	Gozalbo Rovira, Roberto Vicente
dc.contributor.author	Muñoz Collado, Carlos
dc.contributor.author	Buesa Gómez, Javier
dc.contributor.author	Sánchez, Gloria
dc.contributor.author	Rodríguez Díaz, Jesús
dc.date.accessioned	2020-06-09T07:49:14Z
dc.date.available	2020-06-09T07:49:14Z
dc.date.issued	2020
dc.identifier.citation	Santiso Bellón, Cristina Randazzo, Walter Pérez-Cataluña, Alba Vila Vicent, Susana Gozalbo Rovira, Roberto Vicente Muñoz Collado, Carlos Buesa Gómez, Javier Sánchez-Moragas, Gloria Rodríguez Díaz, Jesús 2020 Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016-2017) in Valencia (Spain) Microorganisms 8 3 458-1 458-22
dc.identifier.uri	https://hdl.handle.net/10550/74907
dc.description.abstract	The aim of the present study was to perform the molecular epidemiology of rotaviruses and noroviruses detected in sewage samples from a large wastewater facility from the city of Valencia, Spain. A total of 46 sewage samples were collected over a one-year period (September 2016 to September 2017). Norovirus and rotavirus were detected and quantified by RT-qPCR, genotyped by semi-nested RT-PCR and further characterized by sequencing and phylogenetic analyses. Noroviruses and rotaviruses were widely distributed in sewage samples (69.6% for norovirus GI, 76.0% norovirus GII, and 71.7% rotaviruses) and viral loads varied from 4.33 to 5.75 log PCRU/L for norovirus GI, 4.69 to 6.95 log PCRU/L for norovirus GII, and 4.08 to 6.92 log PCRU/L for rotavirus. Overall, 87.5% (28/32) of GI noroviruses could not be genotyped, 6.25% (2/32) of the samples contained GI.2 genotype, and another 6.25% (2/32) were positive for GI.4 genotype. The most common genotype of GII noroviruses was GII.2 (40%, 14/35), followed by GII.6 (8.6%, 3/35) and GII.17 (5.7%, 2/35) while the remaining GII strains could not be typed (45.7%, 16/35). Rotavirus VP4 genotype P[8] was the only one found in 19 out of 33 rotavirus-positive samples (57.7%). G2 was the most prevalent rotavirus VP7 genotype (15.2%, 5/33) followed by G3, G9, and G12, with two positive samples for each genotype (6.1%, 2/33). In one sample both G1 and G2 genotypes were detected simultaneously (3%). The results presented here show that the surveillance of noroviruses and rotaviruses in sewage is useful for the study of their transmission in the population and their molecular epidemiology.
dc.language.iso	eng
dc.relation.ispartof	Microorganisms, 2020, vol. 8, num. 3, p. 458-1-458-22
dc.subject	Virus RNA
dc.subject	Microorganismes
dc.subject	Aigües residuals Microbiologia
dc.title	Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016-2017) in Valencia (Spain)
dc.type	journal article	es_ES
dc.date.updated	2020-06-09T07:49:15Z
dc.identifier.doi	10.3390/microorganisms8030458
dc.identifier.idgrec	139436
dc.rights.accessRights	open access	es_ES