NAGIOS: RODERIC FUNCIONANDO

Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture

dc.contributor.author	Jorda, Adrian
dc.contributor.author	Aldasoro Celaya, Martín
dc.contributor.author	Aldasoro, Constanza
dc.contributor.author	Guerra Ojeda, Sol
dc.contributor.author	Iradi Casal, Antonio
dc.contributor.author	Vila Salinas, José María
dc.contributor.author	Campos Campos, Juan
dc.contributor.author	Vallés Martí, Lilián Soraya
dc.date.accessioned	2020-06-22T07:26:53Z
dc.date.available	2020-06-22T07:26:53Z
dc.date.issued	2020
dc.identifier.citation	Jorda, Adrian Aldasoro Celaya, Martín Aldasoro, Constanza Guerra Ojeda, Sol Iradi Casal, Antonio Vila Salinas, José María Campos Campos, Juan Vallés Martí, Lilián Soraya 2020 Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture International Journal Of Medical Sciences 17 834 843
dc.identifier.uri	https://hdl.handle.net/10550/75151
dc.description.abstract	Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ1-42 in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10-7 M) in astrocytes in primary culture in presence or absence of Aβ1-42 toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ1-42 peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB protein expression, increasing anti-inflammatory PPAR-γ protein expression, preventing Aβ1-42 toxic effects. Aspirin inhibited COX-2 and iNOS without changes in COX-1 expression, increasing anti-oxidant protein (Cu/Zn-SOD and Mn-SOD) expression in presence or absence of Aβ1-42. Taken together, our results show that aspirin, at low doses increases cell viability by decreasing inflammation and oxidative stress, preventing the deleterious effects of the Aβ1-42 peptide on astrocytes in primary culture. The use of low doses of aspirin may be more suitable for Alzheimer's disease.
dc.language.iso	eng
dc.relation.ispartof	International Journal Of Medical Sciences, 2020, vol. 17, p. 834-843
dc.title	Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture
dc.type	journal article	es_ES
dc.date.updated	2020-06-22T07:26:53Z
dc.identifier.doi	10.7150/ijms.40959
dc.identifier.idgrec	139700
dc.rights.accessRights	open access	es_ES