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Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing

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Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing

dc.contributor.author	Palero Pastor, Ferran
dc.contributor.author	García-Merchan, VH.
dc.contributor.author	Abelló, P.
dc.contributor.author	Macpherson, E.
dc.contributor.author	Pascual, M.
dc.date.accessioned	2020-07-20T04:39:02Z
dc.date.available	2020-07-20T04:39:02Z
dc.date.issued	2012	es_ES
dc.identifier.citation	Palero F; Garcia-Merchan VH; Abelló P; Macpherson E; Pascual M (2012). Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing. In: Molecular Ecology Resources Primer Development Consortium et al. (2012). Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2012-31 May 2012. Molecular Ecology Resources, 12 (5), 972 - 974.	es_ES
dc.identifier.uri	https://hdl.handle.net/10550/75258
dc.description.abstract	The portunid crab Liocarcinus depurator is the dominant decapod crustacean present on mud bottoms of the continental shelf of the Mediterranean Sea. In order to better describe its genetic structure, a total of 11 microsatellites were isolated and tested in 28 L. depurator individuals from the Mediterranean locality of Tarragona. Initially, microsatellite isolation was carried out using the FIASCO methodology, but only 2 useful primer pairs could be designed. Given the low yield obtained, an alternative method based on 454 next-generation sequencing was assayed, which provided excellent results and from which another 9 primer pairs gave positive genotyping. All loci tested were polymorphic, and 2 to 24 (mean 10.54) alleles per locus were identified. The observed and expected heterozygosities per locus ranged from 0.214 to 0.926 and from 0.232 to 0.962, respectively. One locus (Ldep10) showed the presence of null alleles according to the Brookfield estimator implemented in MICROCHECKER. Nevertheless, none of the markers deviated significantly from Hardy–Weinberg equilibrium or showed evidence of linkage disequilibrium. This set of 11 markers is being used to study the population structure and genetic diversity of Liocarcinus depurator populations from both Mediterranean and adjacent Atlantic waters.	es_ES
dc.description.abstract	The portunid crab Liocarcinus depurator is the dominant decapod crustacean present on mud bottoms of the continental shelf of the Mediterranean Sea. In order to better describe its genetic structure, a total of 11 microsatellites were isolated and tested in 28 L. depurator individuals from the Mediterranean locality of Tarragona. Initially, microsatellite isolation was carried out using the FIASCO methodology, but only 2 useful primer pairs could be designed. Given the low yield obtained, an alternative method based on 454 next-generation sequencing was assayed, which provided excellent results and from which another 9 primer pairs gave positive genotyping. All loci tested were polymorphic, and 2 to 24 (mean 10.54) alleles per locus were identified. The observed and expected heterozygosities per locus ranged from 0.214 to 0.926 and from 0.232 to 0.962, respectively. One locus (Ldep10) showed the presence of null alleles according to the Brookfield estimator implemented in MICROCHECKER. Nevertheless, none of the markers deviated significantly from Hardy–Weinberg equilibrium or showed evidence of linkage disequilibrium. This set of 11 markers is being used to study the population structure and genetic diversity of Liocarcinus depurator populations from both Mediterranean and adjacent Atlantic waters.	en_US
dc.language.iso	es	es_ES
dc.subject	portunidae	es_ES
dc.subject	decapod crustaceans	es_ES
dc.subject	pyrosequencing	es_ES
dc.subject	SSRs	es_ES
dc.title	Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing	es_ES
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA	es_ES
dc.identifier.doi	10.1111/j.1755-0998.2012.03173.x	es_ES
dc.identifier.idgrec	078297	es_ES