Background: The current study aimed to assess the amount of fluoride released from fluoride-containing dental adhesives and its effect on micro-tensile bond strength (?TBS) and on resistance to demineralisation of dentine and enamel.Material and Methods: Two fluoride-containing dental adhesives, and a fluoride-free adhesive were used as expe-rimental adhesives. After thermal cycling the ?-TBS of adhesives to dentine and the failure mode were assessed. The fluoride release and cross-sectional microhardness (CSMH) of specimens were measured before and after one day, 7 and 28 days of pH-cycling. The data were analysed using one-way ANOVA, Weibull statistics and repeated measures ANOVA. Results: The results indicated a significant difference between the group of FL and both the SE and LBF groups (p?0.001). The CSMH values of both the dentine and enamel underneath the adhesives was reduced at 28 th day of the pH-cycling compared to the baseline (p?0.001). From day 1 to day 28, the released fluoride declined in both the fluoride containing dental adhesives (p?0.001).Conclusions: Based on the results, the released fluoride from dental adhesives may adversely influence the bond strength and durability of the resin/dentine interface. Moreover, the released fluoride didn?t improve the resistance to demineralisation of adjacent enamel and dentine to bond interface.