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Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide

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Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide

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dc.contributor.author Silva, Emmanuel J. es
dc.contributor.author Hecksher, Fernanda es
dc.contributor.author Vieira, Victor T. es
dc.contributor.author Vivan, Rodrigo R. es
dc.contributor.author Duarte, Marco Antonio es
dc.contributor.author Brasil, Sabrina C. es
dc.contributor.author Antunes, Henrique es
dc.date.accessioned 2020-10-23T10:30:14Z
dc.date.available 2020-10-23T10:30:14Z
dc.date.issued 2020 es
dc.identifier.citation Silva, Emmanuel J. ; Hecksher, Fernanda ; Vieira, Victor T. ; Vivan, Rodrigo R. ; Duarte, Marco Antonio ; Brasil, Sabrina C. ; Antunes, Henrique. Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide. En: Journal of Clinical and Experimental Dentistry, 12 6 2020: 533-539 es
dc.identifier.uri https://hdl.handle.net/10550/76070
dc.description.abstract This study evaluated the cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium-hydroxide Sealer Plus. AH Plus was used as a reference for comparison. Cytotoxicity evaluation was performed according to ISO-10993-5 specifications using MTT assay to check the 3T3 cells viability at 1- to 4-week periods. Antibacterial activity was evaluated using a direct contact test against Enterococcus faecalis. Radiopacity, solubility and flow evaluations were performed according to ISO-6876/2012 specifications. Setting time was assessed following the ANSI/ADA-standard-n.57. The pH level was measured at 3,24,48,72, and 168 hours. Data were statistically analyzed using t-test. The signi?cance level adopted was P<0.05. AH Plus and Sealer Plus showed cytotoxic effects after 24 hours and 1 week of manipulation and become noncytotoxic after 2 weeks. No differences were observed in the cytotoxicity of both tested sealers (P>0.05). Direct contact results revealed that both freshly prepared sealers has antibacterial effects against Enterococcus faecalis. However, after 7 days both sealers had lost much of their antibacterial effects. Although AH Plus presented higher radiopacity and flow than Sealer Plus (P<0.05), both sealers showed minimum required values. No differences were observed in the solubility of both sealers (P>0.05). AH Plus showed a higher setting time when compared to Sealer Plus (P<0.05). AH Plus and Sealer Plus showed a neutral pH during all tested periods (P<0.05). It can be concluded that Sealer Plus showed suitable properties to be used as an endodontic sealer, comparable with those obtained by AH Plus. es
dc.title Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/jced.56534 es
dc.type.hasVersion VoR es_ES

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