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BIO Bragg gratings on microfibers for label-free biosensing

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BIO Bragg gratings on microfibers for label-free biosensing

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dc.contributor.author	Juste-Dolz, Augusto
dc.contributor.author	Delgado-Pinar, Martina
dc.contributor.author	Avellà-Oliver, Miquel
dc.contributor.author	Fernández, Estrella
dc.contributor.author	Pastor, Daniel
dc.date.accessioned	2021-01-25T15:50:08Z
dc.date.available	2021-01-25T15:50:08Z
dc.date.issued	2021	es_ES
dc.identifier.citation	A. Juste-Dolz, M. Delgado-Pinar, M. Avella-Oliver, E. Fernández, D. Pastor, M. V. Andrés, Á. Maquieira, "BIO Bragg gratings on microfibers for label-free biosensing", Biosensors & Bioelectronics, Vol. 176, art. 112916, 2021.	es_ES
dc.identifier.uri	https://hdl.handle.net/10550/77527
dc.description.abstract	Discovering nanoscale phenomena to sense biorecognition events introduces new perspectives to exploit nanoscience and nanotechnology for bioanalytical purposes. Here we present Bio Bragg Gratings (BBGs), a novel biosensing approach that consists of diffractive structures of protein bioreceptors patterned on the surface of optical waveguides, and tailored to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. This work addresses the design, fabrication, and optimization of this system by both theoretical and experimental studies to explore the fundamental physicochemical parameters involved. Functional biomolecular gratings are fabricated by microcontact printing on the surface of tapered optical microfibers, and their structural features were characterized. The transduction principle is experimentally demonstrated, and its quantitative bioanalytical prospects are assessed in a representative immunoassay, based on patterned protein probes and selective IgG targets, in label-free conditions. This biosensing system involves appealing perspectives to avoid unwanted signal contributions from non-specific binding, herein investigated in human serum samples. The work also proves how the optical response of the system can be easily tuned, and it provides insights into the relevance of this feature to conceive multiplexed BBG systems capable to perform multiple label-free biorecognition assays in a single device.	es_ES
dc.language.iso	en	es_ES
dc.subject	biosensor	es_ES
dc.subject	diffraction	es_ES
dc.subject	optical microfiber	es_ES
dc.subject	immunoassay	es_ES
dc.subject	non-specific binding	es_ES
dc.subject	label free	es_ES
dc.title	BIO Bragg gratings on microfibers for label-free biosensing	es_ES
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::FÍSICA	es_ES
dc.identifier.doi	10.1016/j.bios.2020.112916	es_ES
dc.accrualmethod	-	es_ES
dc.embargo.terms	2 year	es_ES
dc.relation.projectID	CTQ2016-75749-R	es_ES
dc.relation.projectID	TEC2016-80385-P	es_ES
dc.relation.projectID	PID2019-110877 GB-I00	es_ES
dc.relation.projectID	PDI2019- 104276RB-I00	es_ES
dc.relation.projectID	PROMETEO/2019/048	es_ES
dc.relation.projectID	PROMETEO/2017/103	es_ES