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Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis

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Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis

dc.contributor.author	Núñez-Ramírez, Rafael
dc.contributor.author	Huesa, Juanjo
dc.contributor.author	Bel Cortés, Yolanda
dc.contributor.author	Ferré Manzanero, Juan
dc.contributor.author	Casino Ferrando, Patricia
dc.contributor.author	Arias-Palomo, Ernesto
dc.date.accessioned	2021-03-10T19:31:08Z
dc.date.available	2021-03-10T19:31:08Z
dc.date.issued	2020
dc.identifier.citation	Núñez-Ramírez, Rafael Huesa, Juanjo Bel Cortés, Yolanda Ferré Manzanero, Juan Casino Ferrando, Patricia Arias-Palomo, Ernesto 2020 Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis Nature Communications 11 3974 1 9
dc.identifier.uri	https://hdl.handle.net/10550/78230
dc.description.abstract	Bacillus thuringiensis Vip3 (Vegetative Insecticidal Protein 3) toxins are widely used in biotech crops to control Lepidopteran pests. These proteins are produced as inactive protoxins that need to be activated by midgut proteases to trigger cell death. However, little is known about their three-dimensional organization and activation mechanism at the molecular level. Here, we have determined the structures of the protoxin and the protease-activated state of Vip3Aa at 2.9 Å using cryo-electron microscopy. The reconstructions show that the protoxin assembles into a pyramid-shaped tetramer with the C-terminal domains exposed to the solvent and the N-terminal region folded into a spring-loaded apex that, after protease activation, drastically remodels into an extended needle by a mechanism akin to that of influenza haemagglutinin. These results provide the molecular basis for Vip3 activation and function, and serves as a strong foundation for the development of more efficient insecticidal proteins.
dc.language.iso	eng
dc.relation.ispartof	Nature Communications, 2020, vol. 11, num. 3974, p. 1-9
dc.subject	Biologia
dc.subject	Proteïnes
dc.subject	Biotecnologia
dc.title	Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis
dc.type	journal article	es_ES
dc.date.updated	2021-03-10T19:31:08Z
dc.identifier.doi	10.1038/s41467-020-17758-5
dc.identifier.idgrec	143887
dc.rights.accessRights	open access	es_ES