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Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in a Heliothis virescens resistant colony

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Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in a Heliothis virescens resistant colony

dc.contributor.author	Pinos, Daniel
dc.contributor.author	Chakroun, Maissa
dc.contributor.author	Millán-Leiva, Anabel
dc.contributor.author	Jurat-Fuentes, Juan Luis
dc.contributor.author	Wright, Denis J.
dc.contributor.author	Hernández Martínez, Patricia
dc.contributor.author	Ferré Manzanero, Juan
dc.date.accessioned	2021-03-11T14:52:30Z
dc.date.available	2021-03-11T14:52:30Z
dc.date.issued	2020
dc.identifier.citation	Pinos, Daniel Chakroun, Maissa Millán-Leiva, Anabel Jurat-Fuentes, Juan Luis Wright, Denis J. Hernández Martínez, Patricia Ferré Manzanero, Juan 2020 Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in a Heliothis virescens resistant colony Toxins 12 6 409
dc.identifier.uri	https://hdl.handle.net/10550/78238
dc.description.abstract	The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The main threat to this technology is the evolution of resistance in targeted insect pests and understanding the mechanistic basis of resistance is crucial to deploy the most appropriate strategies for resistance management. In this work, we tested whether alteration of membrane receptors in the insect midgut might explain the >2000-fold Vip3Aa resistance phenotype in a laboratory-selected colony of Heliothis virescens (Vip-Sel). Binding of 125I-labeled Vip3Aa to brush border membrane vesicles (BBMV) from 3rd instar larvae from Vip-Sel was not significantly different from binding in the reference susceptible colony. Interestingly, BBMV from Vip-Sel larvae showed dramatically reduced levels of membrane-bound alkaline phosphatase (mALP) activity, which was further confirmed by a strong downregulation of the membrane-bound alkaline phosphatase 1 (HvmALP1) gene. However, the involvement of HvmALP1 as a receptor for the Vip3Aa protein was not supported by results from ligand blotting and viability assays with insect cells expressing HvmALP1.
dc.language.iso	eng
dc.relation.ispartof	Toxins, 2020, vol. 12, num. 6, p. 409
dc.subject	Insecticides
dc.subject	Proteïnes
dc.subject	Biotecnologia
dc.title	Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in a Heliothis virescens resistant colony
dc.type	journal article	es_ES
dc.date.updated	2021-03-11T14:52:31Z
dc.identifier.doi	10.3390/toxins12060409
dc.identifier.idgrec	143912
dc.rights.accessRights	open access	es_ES