NAGIOS: RODERIC FUNCIONANDO

Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial.

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial.

dc.contributor.author	Romero Alfonso, Atocha
dc.contributor.author	Jantus Lewintre, Eloisa
dc.contributor.author	García-Peláez, Beatriz
dc.contributor.author	Royuela, Ana
dc.contributor.author	Insa Mollá, Amelia
dc.contributor.author	Cruz, Patricia
dc.contributor.author	Collazo, Ana
dc.contributor.author	Pérez Altozano, Javier
dc.contributor.author	Juan Vidal, Óscar José
dc.contributor.author	Diz Taín, Pilar
dc.contributor.author	Cobo, Manuel
dc.contributor.author	Hernández Marín, Berta
dc.contributor.author	Vázquez Estévez, Sergio
dc.contributor.author	Benítez, Gretel
dc.contributor.author	Guirado, Maria
dc.contributor.author	Majem, Margarita
dc.contributor.author	Bernabé, Reyes
dc.contributor.author	Ortega, Ana Laura
dc.contributor.author	Blasco Cordellat, Ana
dc.contributor.author	Bosch Barrera, Joaquim
dc.contributor.author	Jurado, José María
dc.contributor.author	García González, Jorge
dc.contributor.author	Viteri, Santiago
dc.contributor.author	García Girón, Carlos
dc.contributor.author	Massuti Sureda, Bartomeu
dc.contributor.author	López Martín, Ana
dc.contributor.author	Rodríguez Festa, Alejandro
dc.contributor.author	Calabuig-Fariñas, S.
dc.contributor.author	Molina Vila, Miguel Ángel
dc.contributor.author	Provencio Pulla, Mariano
dc.date.accessioned	2021-03-26T16:44:20Z
dc.date.available	2021-03-26T16:44:20Z
dc.date.issued	2021
dc.identifier.citation	Romero Alfonso, Atocha Jantus Lewintre, Eloisa García-Peláez, Beatriz Royuela, Ana Insa Mollá, Amelia Cruz, Patricia Collazo, Ana Pérez Altozano, Javier Juan Vidal, Óscar José Diz Taín, Pilar Cobo, Manuel Hernández Marín, Berta Vázquez Estévez, Sergio Benítez, Gretel Guirado, Maria Majem, Margarita Bernabé, Reyes Ortega, Ana Laura Blasco Cordellat, Ana Bosch Barrera, Joaquim Jurado, José María García González, Jorge Viteri, Santiago García Girón, Carlos Massuti Sureda, Bartomeu López Martín, Ana Rodríguez Festa, Alejandro Calabuig-Fariñas, S. Molina Vila, Miguel Ángel Provencio Pulla, Mariano 2021 Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial. Molecular Oncology 15 1 43 56
dc.identifier.uri	https://hdl.handle.net/10550/78393
dc.description.abstract	Abstract Several platforms for noninvasive EGFR testing are currently used in the clinical setting with sensitivities ranging from 30% to 100%. Prospective studies evaluating agreement and sources for discordant results remain lacking. Herein, seven methodologies including two next-generation sequencing (NGS)-based methods, three high-sensitivity PCR-based platforms, and two FDA-approved methods were compared using 72 plasma samples, from EGFR-mutant non-small-cell lung cancer (NSCLC) patients progressing on a first-line tyrosine kinase inhibitor (TKI). NGS platforms as well as high-sensitivity PCR-based methodologies showed excellent agreement for EGFR-sensitizing mutations (K = 0.80-0.89) and substantial agreement for T790M testing (K = 0.77 and 0.68, respectively). Mutant allele frequencies (MAFs) obtained by different quantitative methods showed an excellent reproducibility (intraclass correlation coefficients 0.86-0.98). Among other technical factors, discordant calls mostly occurred at mutant allele frequencies (MAFs) ≤ 0.5%. Agreement significantly improved when discarding samples with MAF ≤ 0.5%. EGFR mutations were detected at significantly lower MAFs in patients with brain metastases, suggesting that these patients risk for a false-positive result. Our results support the use of liquid biopsies for noninvasive EGFR testing and highlight the need to systematically report MAFs. Keywords: NGS; circulating free DNA; epidermal growth factor receptor; non-small-cell lung cancer; osimertinib; tyrosine kinase inhibitor.
dc.language.iso	eng
dc.relation.ispartof	Molecular Oncology, 2021, vol. 15, num. 1, p. 43-56
dc.subject	Càncer
dc.subject	Pulmons
dc.title	Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial.
dc.type	journal article	es_ES
dc.date.updated	2021-03-26T16:44:20Z
dc.identifier.doi	10.1002/1878-0261.12832
dc.identifier.idgrec	142781
dc.rights.accessRights	open access	es_ES