NAGIOS: RODERIC FUNCIONANDO

Yeast translation elongation factor eIF5A expression is regulated by nutrient availability through different signalling pathways

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Yeast translation elongation factor eIF5A expression is regulated by nutrient availability through different signalling pathways

dc.contributor.author	Barba-Aliaga, Marina
dc.contributor.author	Villarroel-Vicente, Carlos
dc.contributor.author	Stanciu, Alice
dc.contributor.author	Corman, Alba
dc.contributor.author	Martínez Pastor, María Teresa
dc.contributor.author	Alepuz Martínez, Paula
dc.date.accessioned	2021-04-13T16:34:16Z
dc.date.available	2021-04-13T16:34:16Z
dc.date.issued	2021
dc.identifier.citation	Barba-Aliaga, Marina Villarroel-Vicente, Carlos Stanciu, Alice Corman, Alba Martínez Pastor, María Teresa Alepuz Martínez, Paula 2020 Yeast translation elongation factor eIF5A expression is regulated by nutrient availability through different signalling pathways International Journal Of Molecular Sciences 22 219
dc.identifier.uri	https://hdl.handle.net/10550/78604
dc.description.abstract	Translation elongation factor eIF5A binds to ribosomes to promote peptide bonds between problematic amino acids for the reaction like prolines. eIF5A is highly conserved and essential in eukaryotes, which usually contain two similar but differentially expressed paralogue genes. The human eIF5A-1 isoform is abundant and implicated in some cancer types; the eIF5A-2 isoform is absent in most cells but becomes overexpressed in many metastatic cancers. Several reports have connected eIF5A and mitochondria because it co-purifies with the organelle or its inhibition reduces respiration and mitochondrial enzyme levels. However, the mechanisms of eIF5A mitochondrial function, and whether eIF5A expression is regulated by the mitochondrial metabolism, are unknown. We analysed the expression of yeast eIF5A isoforms Tif51A and Tif51B under several metabolic conditions and in mutants. The depletion of Tif51A, but not Tif51B, compromised yeast growth under respiration and reduced oxygen consumption. Tif51A expression followed dual positive regulation: by high glucose through TORC1 signalling, like other translation factors, to promote growth and by low glucose or non-fermentative carbon sources through Snf1 and heme-dependent transcription factor Hap1 to promote respiration. Upon iron depletion, Tif51A was down-regulated and Tif51B up-regulated. Both were Hap1-dependent. Our results demonstrate eIF5A expression regulation by cellular metabolic status.
dc.language.iso	eng
dc.relation.ispartof	International Journal Of Molecular Sciences, 2020, vol. 22, num. 219
dc.subject	Expressió genètica
dc.subject	Biotecnologia
dc.title	Yeast translation elongation factor eIF5A expression is regulated by nutrient availability through different signalling pathways
dc.type	journal article	es_ES
dc.date.updated	2021-04-13T16:34:17Z
dc.identifier.doi	10.3390/ijms22010219
dc.identifier.idgrec	144609
dc.rights.accessRights	open access	es_ES