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The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation

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The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation

dc.contributor.author	Crespo Salvador, Óscar
dc.contributor.author	Sánchez-Giménez, Lorena
dc.contributor.author	López-Galiano, María José
dc.contributor.author	Fernández-Crespo, Emma
dc.contributor.author	Scalschi, Loredana
dc.contributor.author	García Robles, Inmaculada
dc.contributor.author	Rausell Segarra, Carolina
dc.contributor.author	Real García, María Dolores
dc.contributor.author	González Bosch, Carmen
dc.date.accessioned	2021-05-25T17:44:19Z
dc.date.available	2021-05-25T17:44:19Z
dc.date.issued	2020
dc.identifier.citation	Crespo Salvador, Óscar Sánchez-Giménez, Lorena López-Galiano, María José Fernández-Crespo, Emma Scalschi, Loredana García Robles, Inmaculada Rausell Segarra, Carolina Real García, María Dolores González Bosch, Carmen 2020 The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation Plants-Basel 9 300 1 17
dc.identifier.uri	https://hdl.handle.net/10550/79413
dc.description.abstract	Research into the relationship between epigenetic regulation and resistance to biotic stresses provides alternatives for plant protection and crop improvement. To unravel the mechanisms underlying tomato responses to Botrytis cinerea, we performed a chromatin immunoprecipitation (ChIP) analysis showing the increase in H3K9ac mark along the early induced genes SlyDES, SlyDOX1, and SlyLoxD encoding oxylipin-pathway enzymes, and SlyWRKY75 coding for a transcriptional regulator of hormonal signaling. This histone mark showed a more distinct distribution than the previously studied H3K4me3. The RNAPol-ChIP analysis reflected the actual gene transcription associated with increased histone modifications. A different pattern of marks in the oxylipin-related genes against P. syringae supported a pathogen-specific profile, while no significant differences occurred in SlyWRKY75. The epigenetic regulation of SlyWRKY75 by the intron-binding miR1127-3p was supported by the presence of SlyWRKY75 pre-mRNA in control plants. Interestingly, mRNA was found to be accumulated in response to B. cinerea and P. syringae, while reduction in miRNA only occurred against B. cinerea. The intronic region presented a similar pattern of marks than the rest of the gene in both pathosystems, except for H3K4me3 in the miRNA binding site upon B. cinerea. We located the gene encoding Sly-miR1127-3p, which presented reduced H3K4me3 on its promoter against B. cinerea.
dc.language.iso	eng
dc.relation.ispartof	Plants-Basel, 2020, vol. 9, num. 300, p. 1-17
dc.subject	Fongs patògens
dc.subject	Tomàquets
dc.subject	RNA
dc.title	The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation
dc.type	journal article	es_ES
dc.date.updated	2021-05-25T17:44:19Z
dc.identifier.doi	10.3390/plants9030300
dc.identifier.idgrec	146992
dc.rights.accessRights	open access	es_ES