NAGIOS: RODERIC FUNCIONANDO

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

dc.contributor.author	Rubio-Del-Campo, Antonio
dc.contributor.author	Gozalbo Rovira, Roberto Vicente
dc.contributor.author	Moya-Gonzálvez, Eva M.
dc.contributor.author	Alberola Enguídanos, Juan
dc.contributor.author	Rodríguez Díaz, Jesús
dc.contributor.author	Yebra Yebra, María Jesús
dc.date.accessioned	2021-07-16T13:47:24Z
dc.date.available	2021-07-16T13:47:24Z
dc.date.issued	2021
dc.identifier.citation	Rubio-Del-Campo, Antonio Gozalbo Rovira, Roberto Vicente Moya-Gonzálvez, Eva M. Alberola Enguídanos, Juan Rodríguez Díaz, Jesús Yebra Yebra, María Jesús 2021 Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice Gut Microbes 13 1:e1914377 1 20
dc.identifier.uri	https://hdl.handle.net/10550/79964
dc.description.abstract	Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development. A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions. 16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups. Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides. An increment of the relative abundance of Coprococcus genus was only observed with 3FN. As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase. OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation. The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol. 3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue. The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
dc.language.iso	eng
dc.relation.ispartof	Gut Microbes, 2021, vol. 13, num. 1:e1914377, p. 1-20
dc.subject	Microbiologia
dc.subject	Infants Malalties
dc.title	Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice
dc.type	journal article	es_ES
dc.date.updated	2021-07-16T13:47:24Z
dc.identifier.doi	10.1080/19490976.2021.1914377
dc.identifier.idgrec	147326
dc.rights.accessRights	open access	es_ES