Fatigue causes kinematics modifications during running, and it could be related to injuries. The aim was to identify and compare the effects of central and peripheral fatigue on angular kinematics and spatiotemporal parameters during running. Angular kinematics and spatiotemporal parameters were evaluated using an infrared motion capture system and were registered during 2 min treadmill running in pre- and post-fatigue states in eighteen male recreational runners. Central fatigue was induced by a 30 min running fatigue protocol on a treadmill, while peripheral fatigue in quadriceps and hamstrings muscles was induced by an isokinetic dynamometer fatigue protocol. Central fatigue increased the anterior shank oscillation during the initial contact, knee flexion during the maximum absorption, posterior shank oscillation during propulsion, and stance time (p < 0.05). Peripheral fatigue decreased ankle dorsiflexion during initial contact and increased knee flexion and posterior shank oscillation during propulsion (p < 0.05). Moreover, central fatigue increased to a greater extent the hip and knee flexion and ankle dorsiflexion during initial contact and maximum absorption as well as stance time and propulsion time (p < 0.05). These results suggested that central fatigue causes greater increases in the range of movements during the midstance than peripheral fatigue.