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Microbiota depletion promotes human rotavirus replication in an adult mouse model

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Microbiota depletion promotes human rotavirus replication in an adult mouse model

dc.contributor.author	Gozalbo Rovira, Roberto Vicente
dc.contributor.author	Santiso Bellón, Cristina
dc.contributor.author	Buesa Gómez, Javier
dc.contributor.author	Rubio-Del-Campo, Antonio
dc.contributor.author	Vila Vicent, Susana
dc.contributor.author	Muñoz Collado, Carlos
dc.contributor.author	Yebra Yebra, María Jesús
dc.contributor.author	Monedero García, Vicente
dc.contributor.author	Rodríguez Díaz, Jesús
dc.date.accessioned	2021-09-17T13:00:14Z
dc.date.available	2021-09-17T13:00:14Z
dc.date.issued	2021
dc.identifier.citation	Gozalbo Rovira, Roberto Vicente Santiso Bellón, Cristina Buesa Gómez, Javier Rubio-Del-Campo, Antonio Vila Vicent, Susana Muñoz Collado, Carlos Yebra Yebra, María Jesús Monedero García, Vicente Rodríguez Díaz, Jesús 2021 Microbiota depletion promotes human rotavirus replication in an adult mouse model Biomedicines 9 (7):846 1 14
dc.identifier.uri	https://hdl.handle.net/10550/80316
dc.description.abstract	Intestinal microbiota-virus-host interaction has emerged as a key factor in mediating enteric virus pathogenicity. With the aim of analyzing whether human gut bacteria improve the inefficient replication of human rotavirus in mice, we performed fecal microbiota transplant (FMT) with healthy infants as donors in antibiotic-treated mice. We showed that a simple antibiotic treatment, irrespective of FMT, resulted in viral shedding for 6 days after challenge with the human rotavirus G1P[8] genotype Wa strain (RVwa). Rotavirus titers in feces were also significantly higher in antibiotic-treated animals with or without FMT but they were decreased in animals subject to self-FMT, where a partial re-establishment of specific bacterial taxons was evidenced. Microbial composition analysis revealed profound changes in the intestinal microbiota of antibiotic-treated animals, whereas some bacterial groups, including members of Lactobacillus, Bilophila, Mucispirillum, and Oscillospira, recovered after self-FMT. In antibiotic-treated and FMT animals where the virus replicated more efficiently, differences were observed in gene expression of immune mediators, such as IL1β and CXCL15, as well as in the fucosyltransferase FUT2, responsible for H-type antigen synthesis in the small intestine. Collectively, our results suggest that antibiotic-induced microbiota depletion eradicates the microbial taxa that restrict human rotavirus infectivity in mice.
dc.language.iso	eng
dc.relation.ispartof	Biomedicines, 2021, vol. 9, num. (7):846, p. 1-14
dc.subject	Virus RNA
dc.subject	Microbiologia
dc.subject	Antibiòtics
dc.title	Microbiota depletion promotes human rotavirus replication in an adult mouse model
dc.type	journal article	es_ES
dc.date.updated	2021-09-17T13:00:14Z
dc.identifier.doi	10.3390/biomedicines9070846
dc.identifier.idgrec	147930
dc.rights.accessRights	open access	es_ES