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A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechanisms

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A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechanisms

dc.contributor.author	Xanthopoulou, Aliki
dc.contributor.author	Montero Pau, Javier
dc.contributor.author	Picó, Belén
dc.contributor.author	Boumpas, Panagiotis
dc.contributor.author	Tsaliki, Eleni
dc.contributor.author	Paris, Harry S.
dc.contributor.author	Tsaftaris, Athanasios
dc.contributor.author	Kalivas, Apostolos
dc.contributor.author	Mellidou, Ifigeneia
dc.contributor.author	Ganopoulos, Ioannis
dc.date.accessioned	2021-11-02T13:59:27Z
dc.date.available	2021-11-02T13:59:27Z
dc.date.issued	2021
dc.identifier.citation	Xanthopoulou, Aliki Montero Pau, Javier Picó, Belén Boumpas, Panagiotis Tsaliki, Eleni Paris, Harry S. Tsaftaris, Athanasios Kalivas, Apostolos Mellidou, Ifigeneia Ganopoulos, Ioannis 2021 A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechanisms Bmc Genomics 22 341
dc.identifier.uri	https://hdl.handle.net/10550/80627
dc.description.abstract	Background: Summer squash (Cucurbita pepo: Cucurbitaceae) are a popular horticultural crop for which there is insufficient genomic and transcriptomic information. Gene expression atlases are crucial for the identification of genes expressed in different tissues at various plant developmental stages. Here, we present the first comprehensive gene expression atlas for a summer squash cultivar, including transcripts obtained from seeds, shoots, leaf stem, young and developed leaves, male and female flowers, fruits of seven developmental stages, as well as primary and lateral roots. Results: In total, 27,868 genes and 2352 novel transcripts were annotated from these 16 tissues, with over 18,000 genes common to all tissue groups. Of these, 3812 were identified as housekeeping genes, half of which assigned to known gene ontologies. Flowers, seeds, and young fruits had the largest number of specific genes, whilst intermediate-age fruits the fewest. There also were genes that were differentially expressed in the various tissues, the male flower being the tissue with the most differentially expressed genes in pair-wise comparisons with the remaining tissues, and the leaf stem the least. The largest expression change during fruit development was early on, from female flower to fruit two days after pollination. A weighted correlation network analysis performed on the global gene expression dataset assigned 25,413 genes to 24 coexpression groups, and some of these groups exhibited strong tissue specificity. Conclusions: These findings enrich our understanding about the transcriptomic events associated with summer squash development and ripening. This comprehensive gene expression atlas is expected not only to provide a global view of gene expression patterns in all major tissues in C. pepo but to also serve as a valuable resource for functional genomics and gene discovery in Cucurbitaceae.
dc.language.iso	eng
dc.relation.ispartof	Bmc Genomics, 2021, vol. 22, num. 341
dc.subject	Expressió genètica
dc.subject	Creixement (Plantes)
dc.subject	RNA
dc.title	A comprehensive RNA-Seq-based gene expression atlas of the summer squash (Cucurbita pepo) provides insights into fruit morphology and ripening mechanisms
dc.type	journal article	es_ES
dc.date.updated	2021-11-02T13:59:27Z
dc.identifier.doi	10.1186/s12864-021-07683-2
dc.identifier.idgrec	148670
dc.rights.accessRights	open access	es_ES