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Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database

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Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database

dc.contributor.author	Pinar-Méndez, Anna
dc.contributor.author	Fernández, Sonia
dc.contributor.author	Baquero, David
dc.contributor.author	Vilaró, Carles
dc.contributor.author	Galofré, Belén
dc.contributor.author	González, Susana
dc.contributor.author	Rodrigo-Torres, Lidia
dc.contributor.author	Arahal, David R.
dc.contributor.author	Macián Rovira, M. Carmen
dc.contributor.author	Ruvira, María A.
dc.contributor.author	Aznar Novella, Rosa
dc.contributor.author	Caudet-Segarra, Laia
dc.contributor.author	Sala-Comorera, Laura
dc.contributor.author	Lucena, Francisco
dc.contributor.author	Blanch, Anicet R.
dc.contributor.author	Garcia-Aljaro, Cristina
dc.date.accessioned	2022-01-11T14:33:43Z
dc.date.available	2022-01-11T14:33:43Z
dc.date.issued	2021
dc.identifier.citation	Pinar-Méndez, Anna Fernández, Sonia Baquero, David Vilaró, Carles Galofré, Belén González, Susana Rodrigo-Torres, Lidia Arahal, David R. Macián Rovira, M. Carmen Ruvira, María A. Aznar Novella, Rosa Caudet-Segarra, Laia Sala-Comorera, Laura Lucena, Francisco Blanch, Anicet R. Garcia-Aljaro, Cristina 2021 Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database Water Research 203 117543
dc.identifier.uri	https://hdl.handle.net/10550/81299
dc.description.abstract	According to the European Directives (UE) 2020/2184 and 2009/54/EC, which establishes the sanitary criteria for water intended for human consumption in Europe, water suitable for human consumption must be free of the bacterial indicators Escherichia coli, Clostridium perfringens and Enterococcus spp. Drinking water is also monitored for heterotrophic bacteria, which are not a human health risk, but can serve as an index of bacteriological water quality. Therefore, a rapid, accurate, and cost-effective method for the identification of these colonies would improve our understanding of the culturable bacteria of drinking water and facilitate the task of water management by treatment facilities. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is potentially such a method, although most of the currently available mass spectral libraries have been developed in a clinical setting and have limited environmental applicability. In this work, a MALDITOF MS drinking water library (DWL) was defined and developed by targeting bacteria present in water intended for human consumption. This database, made up of 319 different bacterial strains, can contribute to the routine microbiological control of either treated drinking water or mineral bottled water carried out by water treatment and distribution operators, offering a faster identification rate compared to a clinical sample-based library. The DWL, made up of 96 bacterial genera, 44 of which are not represented in the MALDI-TOF MS bacterial Bruker Daltonics (BDAL) database, was found to significantly improve the identification of bacteria present in drinking water.
dc.language.iso	eng
dc.relation.ispartof	Water Research, 2021, vol. 203, num. 117543
dc.subject	Bacteris
dc.subject	Aigua potable
dc.subject	Microbiologia
dc.title	Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database
dc.type	journal article	es_ES
dc.date.updated	2022-01-11T14:33:43Z
dc.identifier.doi	10.1016/j.watres.2021.117543
dc.identifier.idgrec	149546
dc.rights.accessRights	open access	es_ES