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Saavedra Tortosa, Genaro; Gimeno Gómez, Alejandro; Martínez Corral, Manuel; Sola Pikabea, Jorge; Sánchez Ortiga, Emilio | |||
Aquest document és un/a article, creat/da en: 2022 | |||
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We report a scanning non-confocal fluorescence microscopy scheme that provides images with optical sectioning and with a lateral resolution that surpasses by a factor of two the diffraction resolution limit. This technique is based on the type-1 microscopy concept combined with patterned illumination. The method does not require the application of phase-shifting or post-processing algorithms and provides artifact-free superresolved 3D images. We have validated the theory by means of experimental data. | |||
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