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Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice

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Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice

dc.contributor.author	Herranz Pérez, Vicente
dc.contributor.author	Nakatani, Jin
dc.contributor.author	Ishii, Masaki
dc.contributor.author	Katada, Toshiaki
dc.contributor.author	García Verdugo, José Manuel
dc.contributor.author	Ohata, Shinya
dc.date.accessioned	2022-04-07T09:21:27Z
dc.date.available	2022-04-07T09:21:27Z
dc.date.issued	2022
dc.identifier.citation	Herranz Pérez, Vicente Nakatani, Jin Ishii, Masaki Katada, Toshiaki García Verdugo, José Manuel Ohata, Shinya 2022 Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice Scientific Reports 12 1 1 15
dc.identifier.uri	https://hdl.handle.net/10550/82207
dc.description.abstract	The fusion protein of uncharacterised zinc finger translocation associated (ZFTA) and effector transcription factor of tumorigenic NF‑κB signalling, RELA (ZFTA‑RELA), is expressed in more than two‑thirds of supratentorial ependymoma (ST‑EPN‑RELA), but ZFTA¿s expression profile and functional analysis in multiciliated ependymal (E1) cells have not been examined. Here, we showed the mRNA expression of mouse Zfta peaks on embryonic day (E) 17.5 in the wholemount of the lateral walls of the lateral ventricle. Zfta was expressed in the nuclei of FoxJ1‑positive immature E1 (pre‑E1) cells in E18.5 mouse embryonic brain. Interestingly, the transcription factors promoting ciliogenesis (ciliary TFs) (e.g., multicilin) and ZFTA‑RELA upregulated luciferase activity using a 5′ upstream sequence of ZFTA in cultured cells. Zftatm1/tm1 knock‑in mice did not show developmental defects or abnormal fertility. In the Zftatm1/tm1 E1 cells, morphology, gene expression, ciliary beating frequency and ependymal flow were unaffected. These results suggest that Zfta is expressed in pre‑E1 cells, possibly under the control of ciliary TFs, but is not essential for ependymal development or flow. This study sheds light on the mechanism of the ZFTA‑RELA expression in the pathogenesis of ST‑EPNRELA: Ciliary TFs initiate ZFTA‑RELA expression in pre‑E1 cells, and ZFTA‑RELA enhances its own expression using positive feedback.
dc.language.iso	eng
dc.relation.ispartof	Scientific Reports, 2022, vol. 12, num. 1, p. 1-15
dc.title	Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice
dc.type	journal article	es_ES
dc.date.updated	2022-04-07T09:21:27Z
dc.identifier.doi	10.1038/s41598-022-05526-y
dc.identifier.idgrec	151249
dc.rights.accessRights	open access	es_ES