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Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes

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Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes

dc.contributor.author	Guijarro Ferrer, Lluís
dc.contributor.author	Martínez Camarena, Álvaro
dc.contributor.author	Chicote, Javier U.
dc.contributor.author	García-España, Antonio
dc.contributor.author	Garcia-España Monsonis, Enrique
dc.contributor.author	Inclán Nafría, Mario Alfonso
dc.contributor.author	Verdejo Viu, Begoña
dc.contributor.author	González García, Jorge
dc.date.accessioned	2022-04-29T13:56:40Z
dc.date.available	2022-04-29T13:56:40Z
dc.date.issued	2021
dc.identifier.citation	Guijarro Ferrer, Lluís Martínez Camarena, Álvaro Chicote, Javier U. García-España, Antonio Garcia-España Monsonis, Enrique Inclán Nafría, Mario Alfonso Verdejo Viu, Begoña González García, Jorge 2021 Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes Molecules 26 13 3957
dc.identifier.uri	https://hdl.handle.net/10550/82471
dc.description.abstract	Nucleic acids are essential biomolecules in living systems and represent one of the main targets of chemists, biophysics, biologists, and nanotechnologists. New small molecules are continuously developed to target the duplex (ds) structure of DNA and, most recently, RNA to be used as therapeutics and/or biological tools. Stimuli-triggered systems can promote and hamper the interaction to biomolecules through external stimuli such as light and metal coordination. In this work, we report on the interaction with ds-DNA and ds-RNA of two aza-macrocycles able to coordinate Zn2+ metal ions and form binuclear complexes. The interaction of the aza-macrocycles and the Zn2+ metal complexes with duplex DNA and RNA was studied using UV thermal and fluorescence indicator displacement assays in combination with theoretical studies. Both ligands show a high affinity for ds-DNA/RNA and selectivity for ds-RNA. The ability to interact with these duplexes is blocked upon Zn2+ coordination, which was confirmed by the low variation in the melting temperature and poor displacement of the fluorescent dye from the ds-DNA/RNA. Cell viability assays show a decrease in the cytotoxicity of the metal complexes in comparison with the free ligands, which can be associated with the observed binding to the nucleic acids.
dc.language.iso	eng
dc.relation.ispartof	Molecules, 2021, vol. 26, num. 13, p. 3957
dc.subject	RNA
dc.subject	Àcids nucleics
dc.title	Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes
dc.type	journal article	es_ES
dc.date.updated	2022-04-29T13:56:41Z
dc.identifier.doi	10.3390/molecules26133957
dc.identifier.idgrec	152082
dc.rights.accessRights	open access	es_ES