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Comparative biological properties and mineralization potential of three endodontic materials for vital pulp therapy: Theracal PT, Theracal LC, and Biodentine on human dental pulp stem cells (hDPSCs)

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Comparative biological properties and mineralization potential of three endodontic materials for vital pulp therapy: Theracal PT, Theracal LC, and Biodentine on human dental pulp stem cells (hDPSCs)

dc.contributor.author	Sanz, José Luis
dc.contributor.author	Soler-Doria, Anna
dc.contributor.author	López-García, Sergio
dc.contributor.author	García-Bernal, David
dc.contributor.author	Rodríguez Lozano, Francisco Javier
dc.contributor.author	Lozano, Adrián
dc.contributor.author	Llena Puy, Maria Carmen
dc.contributor.author	Forner Navarro, Leopoldo
dc.contributor.author	Guerrero Gironés, Julia
dc.contributor.author	Melo almiñana, María Pilar
dc.date.accessioned	2022-05-10T07:10:17Z
dc.date.available	2022-05-10T07:10:17Z
dc.date.issued	2021
dc.identifier.citation	Sanz, José Luis Soler-Doria, Anna López-García, Sergio García-Bernal, David Rodríguez Lozano, Francisco Javier Lozano, Adrián Llena Puy, Maria Carmen Forner Navarro, Leopoldo Guerrero Gironés, Julia Melo almiñana, María Pilar 2021 Comparative biological properties and mineralization potential of three endodontic materials for vital pulp therapy: Theracal PT, Theracal LC, and Biodentine on human dental pulp stem cells (hDPSCs) Journal of Endodontics 47 12 1896
dc.identifier.uri	https://hdl.handle.net/10550/82681
dc.description.abstract	Introduction: The aim of this study was to assess the biological properties and mineralization potential of the new Theracal PT (Bisco Inc, Schaumburg, IL) compared with its predecessor Theracal LC (Bisco Inc) and the hydraulic silicate-based cement Biodentine (Septodont, Saint-Maur-des-Fosses, France) on human dental pulp stem cells (hDPSCs) in vitro. Methods: Standardized sample discs were obtained for each material (n 5 30) together with 1:1, 1:2, and 1:4 material eluates. Previously characterized hDPSCs were cultured with the different materials in standardized conditions, and the following assays were performed: a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, a wound healing assay, Annexin-V-FITC and 7-AAD staining (BD Biosciences, San Jose, CA), reactive oxygen species production analysis, cell adhesion and morphology evaluation via scanning electron microscopy and immunofluorescence, quantification of the expression of osteo/odontogenic markers via real-time quantitative reverse-transcriptase polymerase chain reaction, and alizarin red S staining. Statistical significance was established at P , .05. Results: All of the tested dilutions of Theracal LC exhibited a significantly higher cytotoxicity and reactive oxygen species production (P , .001) and a lower cell migration rate than the control group (hDPSCs cultured in growth medium without material extracts) at all of the measured time points (P , .001). Both 1:4 Theracal PT and Biodentine-treated hDPSCs exhibited similar levels of cytocompatibility to that of the control group, a significant up-regulation of at least 1 odontogenic marker (Biodentine: dentin sialophosphoprotein (P , .05); Theracal PT: osteonectin and runt-related transcription factor 2 [P , .001]), and a significantly higher mineralized nodule formation (P , .001). Conclusions: The newly introduced TheraCal PT offers an improved in vitro cytocompatibility and mineralization potential on hDPSCs compared with its predecessor, TheraCal LC, and comparable biological properties to Biodentine
dc.language.iso	eng
dc.relation.ispartof	Journal of Endodontics, 2021, vol. 47, num. 12, p. 1896
dc.subject	Odontologia
dc.title	Comparative biological properties and mineralization potential of three endodontic materials for vital pulp therapy: Theracal PT, Theracal LC, and Biodentine on human dental pulp stem cells (hDPSCs)
dc.type	journal article	es_ES
dc.date.updated	2022-05-10T07:10:17Z
dc.identifier.doi	10.1016/j.joen.2021.08.001
dc.identifier.idgrec	148073
dc.rights.accessRights	open access	es_ES