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Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow

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Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow

dc.contributor.author	Rodríguez Lozano, Francisco Javier
dc.contributor.author	López-García, Sergio
dc.contributor.author	García-Bernal, David
dc.contributor.author	Tomás-Catalá, C.J.
dc.contributor.author	Santos, J.M.
dc.contributor.author	Llena Puy, María Carmen
dc.contributor.author	Lozano, A.
dc.contributor.author	Murcia, L.
dc.contributor.author	Forner Navarro, Leopoldo
dc.date.accessioned	2022-05-16T10:55:41Z
dc.date.available	2022-05-16T10:55:41Z
dc.date.issued	2020
dc.identifier.citation	Rodríguez Lozano, Francisco Javier López-García, Sergio García-Bernal, David Tomás-Catalá, C.J. Santos, J.M. Llena Puy, María Carmen Lozano, A. Murcia, L. Forner Navarro, Leopoldo 2020 Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow International Endodontic Journal 53 9 1216 1228
dc.identifier.uri	https://hdl.handle.net/10550/82781
dc.description.abstract	Aim To evaluate in a laboratory setting the effects of Endosequence BC Sealer HiFlow (Brasseler USA, Savannah, GA, USA), a novel calcium silicate-based sealer developed for use in warm canal filling techniques, on human periodontal ligament stem cells (hPDLSCs). Methodology Eluates of EndoSequence BC Sealer HiFlow (BCHiF) (Brasseler USA), EndoSequence BC Sealer (BCS) (Brasseler USA) and AH Plus (AHP) (Dentsply DeTrey GmbH, Konstanz, Germany), were placed in contact with hPDLSCs. The characterisation of the chemical elements of the root canal sealers was assessed using Scanning Electron Microscopy and Energy Dispersive X-ray analysis (SEM-EDX). Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to determine the ion release of the sealers. MTT assay and wound healing techniques were used to determine cell viability and migration, respectively. Cell morphology and cell attachment were assessed using a direct contact technique of hPDLSCs onto the surface of the sealers and analysed by SEM. The bioactivity potential was carried out with the Alizarin Red and qPCR testing methods. The statistical differences were evaluated using one-way ANOVA and Tukey´s test (p<0.05). Results ICP-MS and EDX revealed significantly more zirconium in BCHiF than BCS (p<0.05), whereas BCS had slightly higher levels of Ca2+ than BCHiF (p<0.05). The cell viability assay revealed no relevant differences between BCS and BCHiF when compared with the control group (p>0.05). Both BCS and BCHiF had similar rates of cell migration to the control group at 24 and 48 hours. Cell morphology and adhesion capacity were also similar for BCS and BCHiF groups, while the AHP group was associated with reduced adhesion capacity. The Alizarin Red assay revealed a significant difference between the BCS and the control group (p<0.001), as well as for the BCHiF group (p<0.001). Finally, BCS and BCHiF promoted overexpression of osteo/cementogenic genes. Conclusions In general, EndoSequence BC Sealer HiFlow possesses suitable biological properties to be safely used as a root canal filling material and promote increased expression of oste/cementogenic genes by hPDLSCs.
dc.language.iso	eng
dc.relation.ispartof	International Endodontic Journal, 2020, vol. 53, num. 9, p. 1216-1228
dc.subject	Odontologia
dc.title	Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow
dc.type	journal article	es_ES
dc.date.updated	2022-05-16T10:55:42Z
dc.identifier.idgrec	145690
dc.rights.accessRights	open access	es_ES