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Multicompartmental Lipopolyplex as vehicle for antigens and genes delivery in vaccine formulations

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Multicompartmental Lipopolyplex as vehicle for antigens and genes delivery in vaccine formulations

dc.contributor.author	Sanmartín Santos, Isaías
dc.contributor.author	Sendra Gisbert, Luis
dc.contributor.author	Moret, Inés
dc.contributor.author	Herrero Cervera, María José
dc.contributor.author	Aliño Pellicer, Salvador F.
dc.date.accessioned	2022-05-18T13:41:13Z
dc.date.available	2022-05-18T13:41:13Z
dc.date.issued	2021
dc.identifier.citation	Sanmartín Santos, Isaías Sendra Gisbert, Luis Moret, Inés Herrero Cervera, María José Aliño Pellicer, Salvador F. 2021 Multicompartmental Lipopolyplex as vehicle for antigens and genes delivery in vaccine formulations Pharmaceutics 13 2 281
dc.identifier.uri	https://hdl.handle.net/10550/82830
dc.description.abstract	Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. The vector characterization and optimization were carried out using liposomes with entrapped carboxyfluorescein and adapted electrophoretic assays. Two types of lipopolyplexes (containing hydrophilic or lipophilic antigens) were employed to evaluate their interest in vaccination. The lipopolyplex loaded with an extract of water-soluble melanoma proteins proved to efficiently induce humoral response in murine melanoma model, increasing the levels of IgM and IgG. The specificity of the immune response induced by the lipopolyplex was demonstrated in mice with the lipopolyplex containing the GD3 ganglioside lipid antigen, abundant in melanoma cells. The levels of anti-GD3 IgG increased markedly without modifying the expression of humoral antibodies against other gangliosides.
dc.language.iso	eng
dc.relation.ispartof	Pharmaceutics, 2021, vol. 13, num. 2, p. 281
dc.subject	Melanoma
dc.subject	Vacunació
dc.subject	Farmacologia
dc.title	Multicompartmental Lipopolyplex as vehicle for antigens and genes delivery in vaccine formulations
dc.type	journal article	es_ES
dc.date.updated	2022-05-18T13:41:14Z
dc.identifier.doi	10.3390/pharmaceutics13020281
dc.identifier.idgrec	152196
dc.rights.accessRights	open access	es_ES