NAGIOS: RODERIC FUNCIONANDO

Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

dc.contributor.author	Duréndez Sáez, Elena
dc.contributor.author	Calabuig Fariñas, Silvia
dc.contributor.author	Torres Martínez, Susana
dc.contributor.author	Moreno Manuel, Andrea
dc.contributor.author	Herreros Pomares, Alejandro
dc.contributor.author	Escorihuela, Eva
dc.contributor.author	Mosqueda, Marais
dc.contributor.author	Gallach, Sandra
dc.contributor.author	Guijarro Jorge, Ricardo
dc.contributor.author	Serna García, Eva
dc.contributor.author	Suárez Cabrera, Cristian
dc.contributor.author	Paramio, Jesús M.
dc.contributor.author	Blasco, Ana
dc.contributor.author	Camps Herrero, Carlos
dc.contributor.author	Jantus Lewintre, Eloisa
dc.date.accessioned	2022-07-19T17:44:41Z
dc.date.available	2022-07-19T17:44:41Z
dc.date.issued	2022
dc.identifier.citation	Duréndez Sáez, Elena Calabuig Fariñas, Silvia Torres Martínez, Susana Moreno Manuel, Andrea Herreros Pomares, Alejandro Escorihuela, Eva Mosqueda, Marais Gallach, Sandra Guijarro Jorge, Ricardo Serna García, Eva Suárez Cabrera, Cristian Paramio, Jesús M. Blasco, Ana Camps Herrero, Carlos Jantus Lewintre, Eloisa 2022 Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer Cancers 14 3216 3216 3239
dc.identifier.uri	https://hdl.handle.net/10550/83470
dc.description.abstract	Lung cancer is a malignant disease with high mortality and poor prognosis, frequently diagnosed at advanced stages. Nowadays, immense progress in treatment has been achieved. However, the present scenario continues to be critical, and a full comprehension of tumor progression mechanisms is required, with exosomes being potentially relevant players. Exosomes are membranous vesicles that contain biological information, which can be transported cell-to-cell and modulate relevant processes in the hallmarks of cancer. The present research aims to characterize the exosomes' cargo and study their role in NSCLC to identify biomarkers. We analyzed exosomes secreted by primary cultures and cell lines, grown in monolayer and tumorsphere formations. Exosomal DNA content showed molecular alterations, whereas RNA high-throughput analysis resulted in a pattern of differentially expressed genes depending on histology. The most significant differences were found in XAGE1B, CABYR, NKX2-1, SEPP1, CAPRIN1, and RIOK3 genes when samples from two independent cohorts of resected NSCLC patients were analyzed. We identified and validated biomarkers for adenocarcinoma and squamous cell carcinoma. Our results could represent a relevant contribution concerning exosomes in clinical practice, allowing for the identification of biomarkers that provide information regarding tumor features, prognosis and clinical behavior of the disease. Keywords: non-small cell lung cancer; liquid biopsy; exosomes; extracellular vesicles; cell cultures; adenocarcinoma; squamous cell carcinoma; biomarker; tumorspheres
dc.relation.ispartof	Cancers, 2022, vol. 14, num. 3216, p. 3216-3239
dc.subject	Càncer
dc.subject	Pulmons
dc.title	Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer
dc.type	journal article	es_ES
dc.date.updated	2022-07-19T17:44:42Z
dc.identifier.doi	10.3390/cancers14133216
dc.identifier.idgrec	154246
dc.rights.accessRights	open access	es_ES