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Denaturing for nanoarchitectonics: local and periodic UV-laser photodeactivation of protein biolayers to create functional patterns for biosensing

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Denaturing for nanoarchitectonics: local and periodic UV-laser photodeactivation of protein biolayers to create functional patterns for biosensing

dc.contributor.author	Juste-Dolz, Augusto
dc.contributor.author	Delgado-Pinar, Martina
dc.contributor.author	Avellà-Oliver, Miquel
dc.contributor.author	Fernández, Estrella
dc.contributor.author	Cruz Muñoz, José Luis
dc.contributor.author	Andrés, Miguel V.
dc.contributor.author	Maquieira, Ángel
dc.date.accessioned	2022-09-28T13:59:27Z
dc.date.available	2022-09-28T13:59:27Z
dc.date.issued	2022
dc.identifier.citation	Juste-Dolz, Augusto Delgado-Pinar, Martina Avellà-Oliver, Miquel Fernández, Estrella Cruz Muñoz, José Luis Andrés, Miguel V. Maquieira, Ángel 2022 Denaturing for nanoarchitectonics: local and periodic UV-laser photodeactivation of protein biolayers to create functional patterns for biosensing Acs Applied Materials & Interfaces 14 36 41640 41648
dc.identifier.uri	https://hdl.handle.net/10550/84047
dc.description.abstract	The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein, we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning principle has proven to be an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns (2 min, 15 × 1.2 mm, relative standard deviation ≃ 16%). This work also investigates the implementation of these protein patterns as transducers for diffractive biosensing. Using a model immunoassay, these patterns have demonstrated negligible signal contributions from non-specific bindings and comparable experimental limits of detection in buffer media and in human serum (53 and 36 ng·mL−1 of unlabeled IgG, respectively).
dc.language.iso	eng
dc.relation.ispartof	Acs Applied Materials & Interfaces, 2022, vol. 14, num. 36, p. 41640-41648
dc.subject	Materials nanoestructurats
dc.subject	Física
dc.title	Denaturing for nanoarchitectonics: local and periodic UV-laser photodeactivation of protein biolayers to create functional patterns for biosensing
dc.type	journal article	es_ES
dc.date.updated	2022-09-28T13:59:27Z
dc.identifier.doi	10.1021/acsami.2c12808
dc.identifier.idgrec	154769
dc.rights.accessRights	open access	es_ES