NAGIOS: RODERIC FUNCIONANDO

Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion

dc.contributor.author	Santiso Bellón, Cristina
dc.contributor.author	Gozalbo Rovira, Roberto Vicente
dc.contributor.author	Buesa Gómez, Javier
dc.contributor.author	Rubio-Del-Campo, Antonio
dc.contributor.author	Peña-Gil, Nazaret
dc.contributor.author	Navarro Lleó, Noemí
dc.contributor.author	Cárcamo-Calvo, Roberto
dc.contributor.author	Yebra Yebra, María Jesús
dc.contributor.author	Monedero García, Vicente
dc.contributor.author	Rodríguez Díaz, Jesús
dc.date.accessioned	2022-10-04T17:29:48Z
dc.date.available	2022-10-04T17:29:48Z
dc.date.issued	2022
dc.identifier.citation	Santiso Bellón, Cristina Gozalbo Rovira, Roberto Vicente Buesa Gómez, Javier Rubio-Del-Campo, Antonio Peña-Gil, Nazaret Navarro Lleó, Noemí Cárcamo-Calvo, Roberto Yebra Yebra, María Jesús Monedero García, Vicente Rodríguez Díaz, Jesús 2022 Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion International Journal Of Molecular Sciences 23 18 10643
dc.identifier.uri	https://hdl.handle.net/10550/84115
dc.description.abstract	Human noroviruses (HuNoVs) are the main cause of acute gastroenteritis causing more than 50,000 deaths per year. Recent evidence shows that the gut microbiota plays a key role in enteric virus infectivity. In this context, we tested whether microbiota depletion or microbiota replacement with that of human individuals susceptible to HuNoVs infection could favor viral replication in mice. Four groups of mice (n = 5) were used, including a control group and three groups that were treated with antibiotics to eliminate the autochthonous intestinal microbiota. Two of the antibiotic-treated groups received fecal microbiota transplantation from a pool of feces from infants (age 1-3 months) or an auto-transplantation with mouse feces that obtained prior antibiotic treatment. The inoculation of the different mouse groups with a HuNoVs strain (GII.4 Sydney [P16] genotype) showed that the virus replicated more efficiently in animals only treated with antibiotics but not subject to microbiota transplantation. Viral replication in animals receiving fecal microbiota from newborn infants was intermediate, whereas virus excretion in feces from auto-transplanted mice was as low as in the control mice. The analysis of the fecal microbiota by 16S rDNA NGS showed deep variations in the composition in the different mice groups. Furthermore, differences were observed in the gene expression of relevant immunological mediators, such as IL4, CXCL15, IL13, TNFα and TLR2, at the small intestine. Our results suggest that microbiota depletion eliminates bacteria that restrict HuNoVs infectivity and that the mechanism(s) could involve immune mediators.
dc.language.iso	eng
dc.relation.ispartof	International Journal Of Molecular Sciences, 2022, vol. 23, num. 18, p. 10643
dc.subject	Virus RNA
dc.subject	Microbiologia
dc.subject	Antibiòtics
dc.title	Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
dc.type	journal article	es_ES
dc.date.updated	2022-10-04T17:29:48Z
dc.identifier.doi	10.3390/ijms231810643
dc.identifier.idgrec	154820
dc.rights.accessRights	open access	es_ES