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Survey of the occurrence of Giardia duodenalis cysts and Cryptosporidium spp. oocysts in green leafy vegetables marketed in the city of Valencia (Spain)

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Survey of the occurrence of Giardia duodenalis cysts and Cryptosporidium spp. oocysts in green leafy vegetables marketed in the city of Valencia (Spain)

dc.contributor.author	Trelis Villanueva, María
dc.contributor.author	Sáez Durán, Sandra
dc.contributor.author	Puchades, Pablo
dc.contributor.author	Castro, Nicole
dc.contributor.author	Miquel, Ana
dc.contributor.author	Gozalbo Monfort, Mónica
dc.contributor.author	Fuentes i Ferrer, Màrius Vicent
dc.date.accessioned	2022-10-07T14:40:59Z
dc.date.available	2022-10-07T14:40:59Z
dc.date.issued	2022
dc.identifier.citation	Trelis Villanueva, María Sáez Durán, Sandra Puchades, Pablo Castro, Nicole Miquel, Ana Gozalbo Monfort, Mónica Fuentes i Ferrer, Màrius Vicent 2022 Survey of the occurrence of Giardia duodenalis cysts and Cryptosporidium spp. oocysts in green leafy vegetables marketed in the city of Valencia (Spain) International Journal of Food Microbiology 379 109847
dc.identifier.uri	https://hdl.handle.net/10550/84141
dc.description.abstract	The role of vegetables usually consumed without prior culinary treatment is known to contribute to the prevalence of foodborne diseases. Cysts and oocysts can contaminate food, which can then be the source of infection in humans. The aim of the study was to assess the occurrence of Giardia duodenalis and Cryptosporidium spp. (oo) cysts in green leafy vegetables marketed in the city of Valencia (Spain) combining parasitological methods, two real-time qPCRs and light microscopy. An experimental field study was conducted on 129 vegetable samples, 64 from conventional farms and 65 from ecological (organic) farms. The samples were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment was used for the search for protozoa. A positive result by both real-time PCRs, or a positive result by one qPCR and confirmation by microscopy was established as a positivity criterion. Giardia duodenalis was detected in 23.0 % of the samples, and Cryptosporidium spp. in 7.8 %. G. duodenalis (41.5 %) and Cryptosporidium spp. (20.0 %) were more frequent in ecological crops. The high level of contamination detected in organic vegetables may be due to the type of fertilizers and the quality of the water used for their irrigation and reinforces the need to take extreme hygiene measures in vegetables that are consumed raw.
dc.language.iso	eng
dc.relation.ispartof	International Journal of Food Microbiology, 2022, vol. 379, p. 109847
dc.subject	Intoxicació alimentària
dc.subject	Paràsits
dc.subject	Aliments Microbiologia
dc.subject	Productes vegetals
dc.title	Survey of the occurrence of Giardia duodenalis cysts and Cryptosporidium spp. oocysts in green leafy vegetables marketed in the city of Valencia (Spain)
dc.type	journal article	es_ES
dc.date.updated	2022-10-07T14:40:59Z
dc.identifier.doi	10.1016/j.ijfoodmicro.2022.109847
dc.identifier.idgrec	154844
dc.rights.accessRights	open access	es_ES