NAGIOS: RODERIC FUNCIONANDO

Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming

dc.contributor.author	Prieto Martínez, Javier
dc.contributor.author	León Rodríguez, Marian
dc.contributor.author	Ponsoda i Martí, Xavier
dc.contributor.author	Sendra Pérez, Ramón
dc.contributor.author	Bort Martí, Roque
dc.contributor.author	Ferrer-Lorente, Raquel
dc.contributor.author	Raya, Angel
dc.contributor.author	López García, Carlos
dc.contributor.author	Torres Ibáñez, José Manuel
dc.date.accessioned	2022-10-21T14:13:44Z
dc.date.available	2022-10-21T14:13:44Z
dc.date.issued	2016
dc.identifier.citation	Prieto Martínez, Javier León Rodríguez, Marian Ponsoda i Martí, Xavier Sendra Pérez, Ramón Bort Martí, Roque Ferrer-Lorente, Raquel Raya, Angel López García, Carlos Torres Ibáñez, José Manuel 2016 Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming Nature Communications 7 11124 1 13
dc.identifier.uri	https://hdl.handle.net/10550/84239
dc.description.abstract	During the process of reprogramming to induced pluripotent stem (iPS) cells, somatic cells switch from oxidative to glycolytic metabolism, a transition associated with profound mitochondrial reorganization. Neither the importance of mitochondrial remodelling for cell reprogramming, nor the molecular mechanisms controlling this process are well understood. Here, we show that an early wave of mitochondrial fragmentation occurs upon expression of reprogramming factors. Reprogramming-induced mitochondrial fission is associated with a minor decrease in mitochondrial mass but not with mitophagy. The pro-fission factor Drp1 is phosphorylated early in reprogramming, and its knockdown and inhibition impairs both mitochondrial fragmentation and generation of iPS cell colonies. Drp1 phosphorylation depends on Erk activation in early reprogramming, which occurs, at least in part, due to downregulation of the MAP kinase phosphatase Dusp6. Taken together, our data indicate that mitochondrial fission controlled by an Erk-Drp1 axis constitutes an early and necessary step in the reprogramming process to pluripotency.
dc.language.iso	eng
dc.relation.ispartof	Nature Communications, 2016, vol. 7, num. 11124, p. 1-13
dc.subject	Cèl·lules
dc.subject	Mitocondris
dc.title	Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming
dc.type	journal article	es_ES
dc.date.updated	2022-10-21T14:13:46Z
dc.identifier.doi	10.1038/ncomms11124
dc.identifier.idgrec	110638
dc.rights.accessRights	open access	es_ES