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Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture

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Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture

dc.contributor.author	Jordá Vallés, Adrián
dc.contributor.author	Aldasoro Celaya, Martín
dc.contributor.author	Campo Palacio, Ignacio
dc.contributor.author	Vila Salinas, José María
dc.contributor.author	Aldasoro, Constanza
dc.contributor.author	Campos Campos, Juan
dc.contributor.author	Colmena Zaragoza, Carlos Manuel
dc.contributor.author	Singh, Sandeep Kumar
dc.contributor.author	Obrador, Elena
dc.contributor.author	Valles Martí, Lilián Soraya
dc.date.accessioned	2022-10-25T19:32:20Z
dc.date.available	2022-10-25T19:32:20Z
dc.date.issued	2022
dc.identifier.citation	Jordá Vallés, Adrián Aldasoro Celaya, Martín Campo Palacio, Ignacio Vila Salinas, José María Aldasoro, Constanza Campos Campos, Juan Colmena Zaragoza, Carlos Manuel Singh, Sandeep Kumar Obrador, Elena Valles Martí, Lilian Soraya 2022 Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture International Journal Of Molecular Sciences 23
dc.identifier.uri	https://hdl.handle.net/10550/84277
dc.description.abstract	Ranolazine (Rn) is a drug used to treat persistent chronic coronary ischemia. It has also been shown to have therapeutic benefits on the central nervous system and an anti-diabetic effect by lowering blood glucose levels; however, no effects of Rn on cellular sensitivity to insulin (Ins) have been demonstrated yet. The present study aimed to investigate the permissive effects of Rn on the actions of Ins in astrocytes in primary culture. Ins (10−8 M), Rn (10−6 M), and Ins + Rn (10−8 M and 10−6 M, respectively) were added to astrocytes for 24 h. In comparison to control cells, Rn and/or Ins caused modifications in cell viability and proliferation. Rn increased protein expression of Cu/Zn-SOD and the pro-inflammatory protein COX-2 was upregulated by Ins. On the contrary, no significant changes were found in the protein expression of NF-κB and IκB. The presence of Rn produced an increase in p-ERK protein and a significant decrease in COX-2 protein expression. Furthermore, Rn significantly increased the effects of Ins on the expression of p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-γ. In addition, Rn + Ins produced a significant decrease in COX-2 expression. In conclusion, Rn facilitated the effects of insulin on the p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-γ signaling pathways, as well as on the anti-inflammatory and antioxidant effects of the hormone.
dc.language.iso	eng
dc.relation.ispartof	International Journal Of Molecular Sciences, 2022, vol. 23
dc.subject	Antioxidants
dc.subject	Sistema nerviós central Malalties
dc.title	Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture
dc.type	journal article	es_ES
dc.date.updated	2022-10-25T19:32:20Z
dc.identifier.doi	10.3390/ijms231911969
dc.identifier.idgrec	155153
dc.rights.accessRights	open access	es_ES