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Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy

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Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy

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dc.contributor.author	Incardona, Nicolò
dc.contributor.author	Tolosa Ruiz, Ángel
dc.contributor.author	Saavedra Tortosa, Genaro
dc.contributor.author	Martínez Corral, Manuel
dc.contributor.author	Sánchez Ortiga, Emilio
dc.date.accessioned	2022-11-10T13:44:04Z
dc.date.available	2022-11-10T13:44:04Z
dc.date.issued	2023
dc.identifier.citation	Incardona, Nicolò Tolosa, Ángel Saavedra Tortosa, Genaro Martínez Corral, Manuel Sánchez Ortiga, Emilio 2023 Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy Optics and Lasers in Engineering 161 107336
dc.identifier.uri	https://hdl.handle.net/10550/84441
dc.description.abstract	Fourier lightfield microscopy (FLMic) is a powerful technique to record 3D images of thick dynamic samples. Belonging FLMic to the general class of computational imaging techniques, its efficiency is determined by several factors, like the optical system, the calibration process, the reconstruction algorithm, or the computation architecture. In the case of FLMic the calibration and the reconstruction algorithm should be fully adapted to the singular features of the technique. To this end, and concerning the reconstruction, we discard the use of experimental PSFs, and propose the use of a synthetic one, which is calculated on the basis of paraxial optics and taking into account the equal influence of diffraction and pixelation. Using this quite simple PSF, performing the adequate calibration and finally implementing the algorithm in GPU, we demonstrate here the possibility of obtaining 3D images with good results in terms of resolution and strong improvement in terms of computation time. In summary, and aiming to accelerate the widespread of FLMic among microscopy users and researchers, we are proposing a fast protocol fully adapted to FLMic and that is very flexible and robust against any slight misalignment or against the change of any optical element.
dc.language.iso	eng
dc.relation.ispartof	Optics and Lasers in Engineering, 2023, vol. 161, p. 107336
dc.subject	Fourier, Anàlisi de
dc.subject	Microscòpia
dc.title	Fast and robust wave optics-based reconstruction protocol for Fourier lightfield microscopy
dc.type	journal article	es_ES
dc.date.updated	2022-11-10T13:44:04Z
dc.identifier.doi	10.1016/j.optlaseng.2022.107336
dc.identifier.idgrec	155549
dc.rights.accessRights	open access	es_ES