NAGIOS: RODERIC FUNCIONANDO

Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy

dc.contributor.author	Gutiérrez, Brenda Celeste
dc.contributor.author	Ancarola, María Eugenia
dc.contributor.author	Volpato-Rossi, Izadora
dc.contributor.author	Marcilla Díaz, Antonio
dc.contributor.author	Ramírez, Marcel Iván
dc.contributor.author	Rosenzvit, Mara Cecilia
dc.contributor.author	Cucher, Marcela
dc.contributor.author	Poncini, Carolina Verónica
dc.date.accessioned	2022-11-21T13:41:54Z
dc.date.available	2022-11-21T13:41:54Z
dc.date.issued	2022
dc.identifier.citation	Gutiérrez, Brenda Celeste Ancarola, María Eugenia Volpato-Rossi, Izadora Marcilla Diaz, Antonio Ramírez, Marcel Iván Rosenzvit, Mara Cecilia Cucher, Marcela Poncini, Carolina Verónica 2022 Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy Frontiers In Cellular And Infection Microbiology 12 980817
dc.identifier.uri	https://hdl.handle.net/10550/84555
dc.description.abstract	Extracellular vesicles (EVs) include a heterogeneous group of particles. Microvesicles, apoptotic bodies and exosomes are the most characterized vesicles. They can be distinguished by their size, morphology, origin and molecular composition. To date, increasing studies demonstrate that EVs mediate intercellular communication. EVs reach considerable interest in the scientific community due to their role in diverse processes including antigenpresentation, stimulation of anti-tumoral immune responses, tolerogenic or inflammatory effects. In pathogens, EV shedding is well described in fungi, bacteria, protozoan and helminths parasites. For Trypanosoma cruzi EV liberation and protein composition was previously described. Dendritic cells (DCs), among other cells, are key players promoting the immune response against pathogens and also maintaining self-tolerance. In previous reports we have demonstrate that T. cruzi downregulates DCs immunogenicity in vitro and in vivo. Here we analyze EVs from the in vitro interaction between blood circulating trypomastigotes (Tp) and bone-marrow-derived DCs. We found that Tp incremented the number and the size of EVs in cultures with DCs. EVs displayed some exosome markers and intracellular RNA. Protein analysis demonstrated that the parasite changes the DC protein-EV profile. We observed that EVs from the interaction of Tp-DCs were easily captured by unstimulated-DCs in comparison with EVs from DCs cultured without the parasite, and also modified the activation status of LPS-stimulated DCs. Noteworthy, we found protection in animals treated with EVs-DCs+Tp and challenged with T. cruzi lethal infection. Our goal is to go deep into the molecular characterization of EVs from the DCs-Tp interaction, in order to identify mediators for therapeutic purposes.
dc.language.iso	eng
dc.relation.ispartof	Frontiers In Cellular And Infection Microbiology, 2022, vol. 12, p. 980817
dc.subject	Cèl·lules
dc.subject	Immunoteràpia
dc.subject	Microbiologia
dc.title	Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
dc.type	journal article	es_ES
dc.date.updated	2022-11-21T13:41:54Z
dc.identifier.doi	10.3389/fcimb.2022.980817
dc.identifier.idgrec	155668
dc.rights.accessRights	open access	es_ES