NAGIOS: RODERIC FUNCIONANDO

RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway

dc.contributor.author	Chattopadhyay, Shiladitya
dc.contributor.author	García Martínez, José
dc.contributor.author	Haimovich, G.
dc.contributor.author	Fischer, Jonathan
dc.contributor.author	Khwaja, Aya
dc.contributor.author	Barkai, O.
dc.contributor.author	Chuartzman, Silvia Gabriela
dc.contributor.author	Schuldiner, Maya
dc.contributor.author	Elran, Ron
dc.contributor.author	Rosenberg, Miriam I.
dc.contributor.author	Urim, Shira
dc.contributor.author	Deshmukh, Shubham
dc.contributor.author	Bohnsack, Katherine E.
dc.contributor.author	Bohnsack, Markus T.
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Choder, M.
dc.date.accessioned	2022-11-24T14:02:35Z
dc.date.available	2022-11-24T14:02:35Z
dc.date.issued	2022
dc.identifier.citation	Chattopadhyay, Shiladitya García Martínez, José Haimovich, G. Fischer, Jonathan Khwaja, Aya Barkai, O. Chuartzman, Silvia Gabriela Schuldiner, Maya Elran, Ron Rosenberg, Miriam I. Urim, Shira Deshmukh, Shubham Bohnsack, Katherine E. Bohnsack, Markus T. Pérez Ortín, José Enrique Choder, M. 2022 RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway Nature Communications 13 7184 1 17
dc.identifier.uri	https://hdl.handle.net/10550/84574
dc.description.abstract	mRNA level is controlled by factors that mediate both mRNA synthesis and decay, including the 5' to 3' exonuclease Xrn1. Here we show that nucleocytoplasmic shuttling of several yeast mRNA decay factors plays a key role in determining both mRNA synthesis and decay. Shuttling is regulated by RNAcontrolled binding of the karyopherin Kap120 to two nuclear localization sequences (NLSs) in Xrn1, location of one ofwhich is conserved fromyeast to human. The decaying RNA binds and masks NLS1, establishing a link between mRNA decay and Xrn1 shuttling. Preventing Xrn1 import, either by deleting KAP120 or mutating the two Xrn1 NLSs, compromises transcription and, unexpectedly, also cytoplasmic decay, uncovering a cytoplasmic decay pathway that initiates in the nucleus.MostmRNAs are degraded by both pathways - the ratio between them represents a full spectrum. Importantly, Xrn1 shuttling is required for proper responses to environmental changes, e.g., fluctuating temperatures, involving proper changes in mRNA abundance and in cell proliferation rate.
dc.language.iso	eng
dc.relation.ispartof	Nature Communications, 2022, vol. 13, num. 7184, p. 1-17
dc.subject	Genètica molecular
dc.subject	RNA
dc.title	RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway
dc.type	journal article	es_ES
dc.date.updated	2022-11-24T14:02:35Z
dc.identifier.doi	10.1038/s41467-022-34417-z
dc.identifier.idgrec	155762
dc.rights.accessRights	open access	es_ES