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A multiplex PCR for the detection of Vibrio vulnificus hazardous to human and/or animal health from seafood

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A multiplex PCR for the detection of Vibrio vulnificus hazardous to human and/or animal health from seafood

dc.contributor.author	Pérez Roig, Arnau
dc.contributor.author	Carmona-Salido, Héctor
dc.contributor.author	Sanjuán Caro, Eva
dc.contributor.author	Fouz Rodríguez, Belén
dc.contributor.author	Amaro González, Carmen
dc.date.accessioned	2022-12-07T14:04:51Z
dc.date.available	2022-12-07T14:04:51Z
dc.date.issued	2022
dc.identifier.citation	Pérez Roig, Arnau Carmona-Salido, Héctor Sanjuán Caro, Eva Fouz Rodríguez, Belén Amaro González, Carmen 2022 A multiplex PCR for the detection of Vibrio vulnificus hazardous to human and/or animal health from seafood International Journal of Food Microbiology 377 109778
dc.identifier.uri	https://hdl.handle.net/10550/84687
dc.description.abstract	Vibrio vulnificus is a zoonotic pathogen linked to aquaculture that is spreading due to climate change. The pathogen can be transmitted to humans and animals by ingestion of raw shellfish or seafood feed, respectively. The aim of this work was to design and test a new procedure to detect V. vulnificus hazardous to human and/or animal health in food/feed samples. For this purpose, we combined a pre-enrichment step with multiplex PCR using primers for the species and for human and animal virulence markers. In vitro assays with mixed DNA from different Vibrio species and Vibrio cultures showed that the new protocol was 100 % specific with a detection limit of 10 cfu/mL. The protocol was successfully validated in seafood using artificially contaminated live shrimp and proved useful also in pathogen isolation from animals and their ecosystem. In conclusion, this novel protocol could be applied in health risk studies associated with food/feed consumption, as well as in the routine identification and subtyping of V. vulnificus from environmental or clinical samples.
dc.language.iso	eng
dc.relation.ispartof	International Journal of Food Microbiology, 2022, vol. 377, num. 109778
dc.subject	Microorganismes patògens
dc.subject	Microbiologia marina
dc.title	A multiplex PCR for the detection of Vibrio vulnificus hazardous to human and/or animal health from seafood
dc.type	journal article	es_ES
dc.date.updated	2022-12-07T14:04:51Z
dc.identifier.doi	10.1016/j.ijfoodmicro.2022.109778
dc.identifier.idgrec	155855
dc.rights.accessRights	open access	es_ES