Hamstring extensibility has been defined as a factor to diminished dynamic stability and therefore increased risk of injury. The purpose of this study was to analyse the effects of hamstring tightness and fatigue on dynamic stability and agility. Nineteen participants were divided between the normal extensibility group (NEG) (n = 9, 82.2° ± 12.4°) and hamstrings tightness group (HTG) (n = 10, 64° ± 4.9°) using the passive straight leg raise test. To analyse dynamic stability and agility, they performed the modified Star Excursion Balance Test (mSEBT) and Dynamic Postural Stability Index (DPSI), and hexagon agility test, respectively, before and after a fatigue protocol. A two-way repeated measures ANOVA was used to determine differences among conditions: NEG vs. HTG, and rested vs. fatigued. HTG showed a significantly lower reach in the anterior direction in the mSEBT in pre- and post-fatigue than NEG. Participants in the NEG showed poor stability after landing in the mediolateral direction on DPSI post-fatigue. No significant changes were found in agility related with the group nor fatigue state. Participants with hamstring extensibility reduction has no differences in dynamic stability after landing nor agility after fatigue test, but significantly affects reaching distances during one-leg balance. As a conclusion, a reduction in range of motion in HTG was observed, but no other effects were observed on performance and dynamic stability after a local fatigue protocol depending on hamstring extensibility.