NAGIOS: RODERIC FUNCIONANDO

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

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Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

dc.contributor.author	Herranz Pérez, Vicente
dc.contributor.author	García Verdugo, José Manuel
dc.contributor.author	Hall, Emma A.
dc.contributor.author	Kumar, Dhivya
dc.contributor.author	Prosser, Suzanna L.
dc.contributor.author	Yeyati, Patricia L.
dc.contributor.author	Rose, Lorraine
dc.contributor.author	McKie, Lisa
dc.contributor.author	Dodd, Daniel O.
dc.contributor.author	Tennant, Peter A.
dc.contributor.author
dc.date.accessioned	2023-05-02T10:10:49Z
dc.date.available	2023-05-02T10:10:49Z
dc.date.issued	2023
dc.identifier.citation	Herranz Pérez, Vicente García Verdugo, José Manuel Hall, Emma A. Kumar, Dhivya Prosser, Suzanna L. Yeyati, Patricia L. Rose, Lorraine McKie, Lisa Dodd, Daniel O. Tennant, Peter A. 2023 Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis Elife 12 1 36
dc.identifier.uri	https://hdl.handle.net/10550/86265
dc.description.abstract	Centrosomes are orbited by centriolar satellites, dynamic multiprotein assemblies nucleated by Pericentriolar material 1 (PCM1). To study the requirement for centriolar satellites, we generated mice lacking PCM1, a crucial component of satellites. Pcm1−/− mice display partially penetrant perinatal lethality with survivors exhibiting hydrocephalus, oligospermia, and cerebellar hypoplasia, and variably expressive phenotypes such as hydronephrosis. As many of these phenotypes have been observed in human ciliopathies and satellites are implicated in cilia biology, we investigated whether cilia were affected. PCM1 was dispensable for ciliogenesis in many cell types, whereas Pcm1−/− multiciliated ependymal cells and human PCM1−/− retinal pigmented epithelial 1 (RPE1) cells showed reduced ciliogenesis. PCM1−/− RPE1 cells displayed reduced docking of the mother centriole to the ciliary vesicle and removal of CP110 and CEP97 from the distal mother centriole, indicating compromised early ciliogenesis. Similarly, Pcm1−/− ependymal cells exhibited reduced removal of CP110 from basal bodies in vivo. We propose that PCM1 and centriolar satellites facilitate efficient trafficking of proteins to and from centrioles, including the departure of CP110 and CEP97 to initiate ciliogenesis, and that the threshold to trigger ciliogenesis differs between cell types.
dc.language.iso	eng
dc.relation.ispartof	Elife, 2023, vol. 12, p. 1-36
dc.subject	Biologia
dc.title	Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis
dc.type	journal article
dc.date.updated	2023-05-02T10:10:50Z
dc.identifier.doi	10.7554/eLife.79299
dc.identifier.idgrec	157711
dc.rights.accessRights	open access