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RNA-seq transcriptome analysis provides candidate genes for resistance to tomato leaf curl New Delhi virus in melon

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RNA-seq transcriptome analysis provides candidate genes for resistance to tomato leaf curl New Delhi virus in melon

dc.contributor.author	Sáez, Cristina
dc.contributor.author	Flores León, Alejandro
dc.contributor.author	Montero Pau, Javier
dc.contributor.author	Sífres, Alicia
dc.contributor.author	Dhillon, Narinder Pal Singh
dc.contributor.author	López, Carmelo
dc.contributor.author	Picó, Belén
dc.date.accessioned	2023-05-12T11:05:43Z
dc.date.available	2023-05-12T11:05:43Z
dc.date.issued	2022
dc.identifier.citation	Sáez, Cristina Flores León, Alejandro Montero Pau, Javier Sífres, Alicia Dhillon, Narinder Pal Singh López, Carmelo Picó, Belén 2022 RNA-seq transcriptome analysis provides candidate genes for resistance to tomato leaf curl New Delhi virus in melon Frontiers In Plant Science 12 1 26
dc.identifier.uri	https://hdl.handle.net/10550/86572
dc.description.abstract	Tomato leaf curl New Delhi virus (ToLCNDV) emerged in the Mediterranean Basin in 2012 as the first DNA bipartite begomovirus (Geminiviridae family), causing severe yield and economic losses in cucurbit crops. A major resistance locus was identified in the wild melon accession WM-7 (Cucumis melo kachri group), but the mechanisms involved in the resistant response remained unknown. In this work, we used RNA-sequencing to identify disease-associated genes that are differentially expressed in the course of ToLCNDV infection and could contribute to resistance. Transcriptomes of the resistant WM-7 genotype and the susceptible cultivar Piñonet Piel de Sapo (PS) (C. melo ibericus group) in ToLCNDV and mock inoculated plants were compared at four time points during infection (0, 3, 6, and 12 days post inoculation). Different gene expression patterns were observed over time in the resistant and susceptible genotypes in comparison to their respective controls. Differentially expressed genes (DEGs) in ToLCNDV-infected plants were classified using gene ontology (GO) terms, and genes of the categories transcription, DNA replication, and helicase activity were downregulated in WM-7 but upregulated in PS, suggesting that reduced activity of these functions reduces ToLCNDV replication and intercellular spread and thereby contributes to resistance. DEGs involved in the jasmonic acid signaling pathway, photosynthesis, RNA silencing, transmembrane, and sugar transporters entail adverse consequences for systemic infection in the resistant genotype, and lead to susceptibility in PS. The expression levels of selected candidate genes were validated by qRT-PCR to corroborate their differential expression upon ToLCNDV infection in resistant and susceptible melon. Furthermore, single nucleotide polymorphism (SNPs) with an effect on structural functionality of DEGs linked to the main QTLs for ToLCNDV resistance have been identified. The obtained results pinpoint cellular functions and candidate genes that are differentially expressed in a resistant and susceptible melon line in response to ToLCNDV, an information of great relevance for breeding ToLCNDV-resistant melon cultivars.
dc.language.iso	eng
dc.relation.ispartof	Frontiers In Plant Science, 2022, vol. 12, p. 1-26
dc.subject	Microbiologia
dc.subject	Ecologia
dc.subject	Ecologia agrícola
dc.title	RNA-seq transcriptome analysis provides candidate genes for resistance to tomato leaf curl New Delhi virus in melon
dc.type	journal article
dc.date.updated	2023-05-12T11:05:43Z
dc.identifier.doi	10.3389/fpls.2021.798858
dc.identifier.idgrec	158507
dc.rights.accessRights	open access