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Metabolomic Diversity of Human Milk Cells over the Course of Lactation - A Preliminary Study

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Metabolomic Diversity of Human Milk Cells over the Course of Lactation - A Preliminary Study

dc.contributor.author	Moreno Torres, Marta
dc.contributor.author	Ten Doménech, Isabel
dc.contributor.author	Cascant Vilaplana, Mari Merce
dc.contributor.author	Navarro Esteve, Víctor
dc.contributor.author	Felderer, Birgit
dc.contributor.author	Moreno Giménez, Alba
dc.contributor.author	Rienda, Iván
dc.contributor.author	Gormaz, María
dc.contributor.author	Perez Guaita, David
dc.contributor.author	Quintás Soriano, Guillermo
dc.contributor.author	Kuligowski, Julia
dc.date.accessioned	2023-05-18T07:04:00Z
dc.date.available	2023-05-18T07:04:00Z
dc.date.issued	2023
dc.identifier.citation	Moreno Torres, Marta Ten Doménech, Isabel Cascant Vilaplana, Mari Merce Navarro Esteve, Víctor Felderer, Birgit Moreno Giménez, Alba Rienda, Iván Gormaz, María Perez Guaita, David Quintás Soriano, Guillermo Kuligowski, Julia 2023 Metabolomic Diversity of Human Milk Cells over the Course of Lactation - A Preliminary Study Nutrients 15 5 1 15
dc.identifier.uri	https://hdl.handle.net/10550/86690
dc.description.abstract	Human milk (HM) is a complex biofluid containing a wide cell variety including epithelial cells and leukocytes. However, the cellular compositions and their phenotypic properties over the course of lactation are poorly understood. The aim of this preliminary study was to characterize the cellular metabolome of HM over the course of lactation. Cells were isolated via centrifugation and the cellular fraction was characterized via cytomorphology and immunocytochemical staining. Cell metabolites were extracted and analyzed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-QqTOF-MS) in the positive and negative electrospray ionization modes. Immunocytochemical analysis revealed a high variability of the number of detected cells with relative median abundances of 98% of glandular epithelial cells, 1% of leukocytes, and 1% of keratinocytes. Significant correlations between the milk postnatal age with percentage of epithelial cells and leukocytes, and with total cell count were observed. Results from the Hierarchical Cluster Analysis of immunocytochemical profiles were very similar to those observed in the analysis of the metabolomic profiles. In addition, metabolic pathway analysis showed alterations in seven metabolic pathways correlating with postnatal age. This work paves the way for future investigations on changes in the metabolomic fraction of the cellular compartment of HM.
dc.language.iso	eng
dc.relation.ispartof	Nutrients, 2023, vol. 15, num. 5, p. 1-15
dc.subject	Bioquímica
dc.subject	Biologia
dc.subject	Nutrició
dc.title	Metabolomic Diversity of Human Milk Cells over the Course of Lactation - A Preliminary Study
dc.type	journal article
dc.date.updated	2023-05-18T07:04:01Z
dc.identifier.doi	10.3390/nu15051100
dc.identifier.idgrec	158847
dc.rights.accessRights	open access