NAGIOS: RODERIC FUNCIONANDO

Phosphoproteomic analysis accross the yeast life cycle reveals homeostatic regulation of fatty acyl chain-length by phosphorylation of the fatty acid synthase complex

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Phosphoproteomic analysis accross the yeast life cycle reveals homeostatic regulation of fatty acyl chain-length by phosphorylation of the fatty acid synthase complex

dc.contributor.author	Moreno Torres, Marta
dc.contributor.author	Casanovas, Albert
dc.contributor.author	Martínez Montañés, Fernando
dc.contributor.author	Topolska, Magdalena
dc.contributor.author	Marshall, David L.
dc.contributor.author	Sprenger, Richard R.
dc.contributor.author	Poad, Berwyck L.J.
dc.contributor.author	Blanksby, Stephen J.
dc.contributor.author	Hermansson, Martin
dc.contributor.author	Jensen, Ole N.
dc.contributor.author	Ejsing, Christer S.
dc.date.accessioned	2023-05-19T10:04:30Z
dc.date.available	2023-05-19T10:04:30Z
dc.date.issued	2020
dc.identifier.citation	Moreno Torres, Marta Casanovas, Albert Martínez Montañés, Fernando Topolska, Magdalena Marshall, David L. Sprenger, Richard R. Poad, Berwyck L.J. Blanksby, Stephen J. Hermansson, Martin Jensen, Ole N. Ejsing, Christer S. 2020 Phosphoproteomic analysis accross the yeast life cycle reveals homeostatic regulation of fatty acyl chain-length by phosphorylation of the fatty acid synthase complex Cell Reports 32 6 1 25
dc.identifier.uri	https://hdl.handle.net/10550/86705
dc.description.abstract	The ability to remodel lipid metabolism under changing conditions is pivotal for cellular functionality and homeostasis. Here, we characterize the regulatory landscape of phosphorylation-based signaling events across the life cycle of Saccharomyces cerevisiae and determine its impact on the regulation of lipid metabolism. Our data show that 50 lipid metabolic proteins are differentially phosphorylated as cells transit between different physiological states. To identify functional phosphosites, we devised a strategy where multiple phosphosites are simultaneously mutated into phosphomimetic or phosphodeficient alleles and mutants are phenotyped by in-depth lipidomics flux analysis. This uncovers functional phosphosites in the phosphatidate cytidylyltransferase Cds1, the phosphatidylserine synthase Cho1, and Fas2, the α-subunit of the fatty acid synthase (FAS) complex. Furthermore, we show that the fatty acyl chain length produced by FAS is governed by phosphorylation. Overall, our work demonstrates a vital role for phosphoregulation of lipid metabolism and provides a resource to investigate its molecular underpinnings.
dc.language.iso	eng
dc.relation.ispartof	Cell Reports, 2020, vol. 32, num. 6, p. 1-25
dc.subject	Bioquímica
dc.subject	Biologia
dc.title	Phosphoproteomic analysis accross the yeast life cycle reveals homeostatic regulation of fatty acyl chain-length by phosphorylation of the fatty acid synthase complex
dc.type	journal article
dc.date.updated	2023-05-19T10:04:31Z
dc.identifier.doi	10.1016/j.celrep.2020.108024
dc.identifier.idgrec	158854
dc.rights.accessRights	open access