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Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples.

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Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples.

dc.contributor.author	Cuevas Ferrando, Enric
dc.contributor.author	Randazzo, Walter
dc.contributor.author	Pérez Cataluña, Alba
dc.contributor.author	Falcó Ferrando, Irene Lourdes
dc.contributor.author	Navarro Ortega, David
dc.contributor.author	Martin-Latil, Sandra
dc.contributor.author	Sánchez, Gloria
dc.date.accessioned	2023-05-25T13:46:02Z
dc.date.available	2023-05-25T13:46:02Z
dc.date.issued	2021
dc.identifier.citation	Cuevas Ferrando, Enric Randazzo, Walter Pérez Cataluña, Alba Falcó Ferrando, Irene Lourdes Navarro Ortega, David Martin-Latil, Sandra Sánchez, Gloria 2021 Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples. Scientific Reports 11 18120
dc.identifier.uri	https://hdl.handle.net/10550/86946
dc.description.abstract	Isolation, contact tracing and restrictions on social movement are being globally implemented to prevent and control onward spread of SARS-CoV-2, even though the infection risk modelled on RNA detection by RT-qPCR remains biased as viral shedding and infectivity are not discerned. Thus, we aimed to develop a rapid viability RT-qPCR procedure to infer SARS-CoV-2 infectivity in clinical specimens and environmental samples. We screened monoazide dyes and platinum compounds as viability molecular markers on five SARS-CoV-2 RNA targets. A platinum chloride-based viability RT-qPCR was then optimized using genomic RNA, and inactivated SARS-CoV-2 particles inoculated in buffer, stool, and urine. Our results were finally validated in nasopharyngeal swabs from persons who tested positive for COVID-19 and in wastewater samples positive for SARS-CoV-2 RNA. We established a rapid viability RT-qPCR that selectively detects potentially infectious SARS-CoV-2 particles in complex matrices. In particular, the confirmed positivity of nasopharyngeal swabs following the viability procedure suggests their potential infectivity, while the complete prevention of amplification in wastewater indicated either non-infectious particles or free RNA. The viability RT-qPCR approach provides a more accurate ascertainment of the infectious viruses detection and it may complement analyses to foster risk-based investigations for the prevention and control of new or re-occurring outbreaks with a broad application spectrum.
dc.language.iso	eng
dc.relation.ispartof	Scientific Reports, 2021, vol. 11, p. 18120
dc.subject	Aigües residuals
dc.title	Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples.
dc.type	journal article
dc.date.updated	2023-05-25T13:46:03Z
dc.identifier.doi	10.1038/s41598-021-97700-x
dc.identifier.idgrec	159267
dc.rights.accessRights	open access